RRC ID 49381
著者 Lan QX, Chen J, Lin L, Ye XL, Yan QY, Huang JF, Liu CC, Yang GW.
タイトル Sporolactobacillus pectinivorans sp. nov., an anaerobic bacterium isolated from spoiled jelly.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-positive-staining, endospore-forming, facultatively anaerobic, lactic-acid-producing bacterium, strain GD201205T, was isolated from spoiled jelly in China. Strain GD201205T fermented glucose, fructose, mannose, sucrose, raffinose and turanose, but negative for nitrate reduction, catalase and oxidase. The predominant fatty acids of the strain were anteiso-C17 : 0 and anteiso-C15 : 0. Whole-cell hydrolysates contained glycine and alanine with meso-iaminopimelic acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, glycolipid 1 and glycolipid 2. The DNA G+C content of strain GD201205T was 48.7 mol%. 16S rRNA gene sequence analysis indicated that the strain belonged to the genus Sporolactobacillus and was most closely related to Sporolactobacillus vineaeKCTC 5376T and Sporolactobacillus putidusJCM 15325T with 16S rRNA gene sequence similarities of 97.5 and 96.9 %, respectively. Levels of DNA-DNA relatedness between strain GD201205T and Sporolactobacillus vineaeKCTC 5376Tand Sporolactobacillus putidusJCM 15325T were 29.2 and 47.6 %, respectively. Phylogenetic analysis based on the 16S rRNA gene and gyrB gene revealed that strain GD201205T was clearly distinct from all related species of the genus Sporolactobacillus. On the basis of the phylogenetic, chemotaxonomic and phenotypic evidence given in this study, strain GD201205T should be classified as a representative of a novel species of the genus Sporolactobacillus for which the name Sporolactobacillus pectinivorans is proposed. The type strain is GD201205T (=CICC 23867T=KCTC 15488T).
巻・号 66(11)
ページ 4323-4328
公開日 2016-11-1
DOI 10.1099/ijsem.0.001351
PMID 27516092
MeSH Bacillales / classification* Bacillales / genetics Bacillales / isolation & purification Bacteria, Anaerobic / classification Bacteria, Anaerobic / genetics Bacteria, Anaerobic / isolation & purification Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Food Microbiology* Glycolipids / chemistry Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 3
リソース情報
一般微生物 JCM 15325