RRC ID 51763
著者 Hidema S, Fukuda T, Date S, Tokitake Y, Matsui Y, Sasaki H, Nishimori K.
タイトル Transgenic expression of Telomerase reverse transcriptase (Tert) improves cell proliferation of primary cells and enhances reprogramming efficiency into the induced pluripotent stem cell.
ジャーナル Biosci Biotechnol Biochem
Abstract The enzymatic activity of telomerase is important for the extension of the telomere repeat sequence and overcoming cellular senescence. We generated a conditional transgenic mouse line, carrying the telomerase reverse transcriptase (Tert) expression cassette, controlled by the Cre-loxP-mediated recombination. In our study, Cre recombinase expression efficiently activated Tert expression, resulting in its increased enzymatic activity, which extended the period of cellular proliferation until the keratinocytes entered senescence. This suggests that transgenic Tert expression is effective in enhancing primary cell proliferation. Notably, Tert expression increased colony formation of induced pluripotent stem (iPS) cells after the introduction of four reprogramming factors, Oct-4, klf4, SOX-2, and c-Myc into the transgenic fibroblasts. To the best of our knowledge, this is the first study to show that the transgenic Tert expression enhances reprogramming efficiency of iPS cells, which indicates a critical role for Tert in the reprogramming process.
巻・号 80(10)
ページ 1925-33
公開日 2016-10-1
DOI 10.1080/09168451.2016.1191330
PMID 27297181
MeSH Animals Cell Line Cell Proliferation Cellular Reprogramming* Gene Expression Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism* Integrases / metabolism Kruppel-Like Factor 4 Mice Mice, Transgenic Telomerase / genetics*
IF 1.516
引用数 3
リソース情報
ヒト・動物細胞 E14tg2a(AES0135)