RRC ID 44329
著者 Akihisa T, Pan X, Nakamura Y, Kikuchi T, Takahashi N, Matsumoto M, Ogihara E, Fukatsu M, Koike K, Tokuda H.
タイトル Limonoids from the fruits of Melia azedarach and their cytotoxic activities.
ジャーナル Phytochemistry
Abstract Thirty-one limonoids and one tirucallane-type triterpenoid were isolated from the fruits of Melia azedarach (Meliaceae). The structures of 14 of these isolated compounds were elucidated on the basis of spectroscopic analyses and comparison with literature. All of these compounds were evaluated for their cytotoxic activities against HL60, A549, AZ521, and SK-BR-3 human cancer cell lines. Meliarachin C (IC50 0.65 μM) and 3-O-deacetyl-4'-demethyl-28-oxosalannin (IC50 2.8 μM) exhibited potent cytotoxic activity against HL60 cells, and this was demonstrated mainly due to the induction of apoptosis by flow cytometry. Western blot analysis suggested that both compounds induced apoptosis via both the mitochondrial and death receptor-mediated pathways. In addition, 25 compounds were evaluated for their inhibitory effects against the Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells.
巻・号 89
ページ 59-70
公開日 2013-5-1
DOI 10.1016/j.phytochem.2013.01.015
PII S0031-9422(13)00037-X
PMID 23465718
MeSH Antigens, Viral / metabolism Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Antineoplastic Agents / toxicity Apoptosis / drug effects Cell Line, Tumor Fruit / chemistry* Herpesvirus 4, Human / immunology Humans Inhibitory Concentration 50 Limonins / isolation & purification Limonins / pharmacology* Limonins / toxicity Lymphocytes / cytology Lymphocytes / drug effects Melia azedarach / chemistry*
IF 3.044
引用数 33
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞