RRC ID |
44329
|
著者 |
Akihisa T, Pan X, Nakamura Y, Kikuchi T, Takahashi N, Matsumoto M, Ogihara E, Fukatsu M, Koike K, Tokuda H.
|
タイトル |
Limonoids from the fruits of Melia azedarach and their cytotoxic activities.
|
ジャーナル |
Phytochemistry
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Abstract |
Thirty-one limonoids and one tirucallane-type triterpenoid were isolated from the fruits of Melia azedarach (Meliaceae). The structures of 14 of these isolated compounds were elucidated on the basis of spectroscopic analyses and comparison with literature. All of these compounds were evaluated for their cytotoxic activities against HL60, A549, AZ521, and SK-BR-3 human cancer cell lines. Meliarachin C (IC50 0.65 μM) and 3-O-deacetyl-4'-demethyl-28-oxosalannin (IC50 2.8 μM) exhibited potent cytotoxic activity against HL60 cells, and this was demonstrated mainly due to the induction of apoptosis by flow cytometry. Western blot analysis suggested that both compounds induced apoptosis via both the mitochondrial and death receptor-mediated pathways. In addition, 25 compounds were evaluated for their inhibitory effects against the Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells.
|
巻・号 |
89
|
ページ |
59-70
|
公開日 |
2013-5-1
|
DOI |
10.1016/j.phytochem.2013.01.015
|
PII |
S0031-9422(13)00037-X
|
PMID |
23465718
|
MeSH |
Antigens, Viral / metabolism
Antineoplastic Agents / isolation & purification
Antineoplastic Agents / pharmacology*
Antineoplastic Agents / toxicity
Apoptosis / drug effects
Cell Line, Tumor
Fruit / chemistry*
Herpesvirus 4, Human / immunology
Humans
Inhibitory Concentration 50
Limonins / isolation & purification
Limonins / pharmacology*
Limonins / toxicity
Lymphocytes / cytology
Lymphocytes / drug effects
Melia azedarach / chemistry*
|
IF |
3.044
|
引用数 |
33
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|