| RRC ID |
51139
|
| 著者 |
Kim CC, Kelly WJ, Patchett ML, Tannock GW, Jordens Z, Stoklosinski HM, Taylor JW, Sims IM, Bell TJ, Rosendale DI.
|
| タイトル |
Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel anaerobic pectinolytic bacterium (strain 14T) was isolated from human faeces. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 14T belonged to the family Ruminococcaceae, but was located separately from known clostridial clusters within the taxon. The closest cultured relative of strain 14T was Acetivibrio cellulolyticus (89.7 % sequence similarity). Strain 14T shared ~99 % sequence similarity with cloned 16S rRNA gene sequences from uncultured bacteria derived from the human gut. Cells were Gram-stain-positive, non-motile cocci approximately 0.6 µm in diameter. Strain 14T fermented pectins from citrus peel, apple, and kiwifruit as well as carbohydrates that are constituents of pectins and hemicellulose, such as galacturonic acid, xylose, and arabinose. TEM images of strain 14T, cultured in association with plant tissues, suggested extracellular fibrolytic activity associated with the bacterial cells, forming zones of degradation in the pectin-rich regions of middle lamella. Phylogenetic and phenotypic analysis supported the differentiation of strain 14T as a novel genus in the family Ruminococcaceae. The name Monoglobus pectinilyticus gen. nov., sp. nov. is proposed; the type strain is 14T (JCM 31914T=DSM 104782T).
|
| 巻・号 |
67(12)
|
| ページ |
4992-4998
|
| 公開日 |
2017-12-1
|
| DOI |
10.1099/ijsem.0.002395
|
| PMID |
29039307
|
| MeSH |
Adult
Bacterial Typing Techniques
Base Composition
Clostridiales / classification*
Clostridiales / genetics
Clostridiales / isolation & purification
DNA, Bacterial / genetics
Feces / microbiology*
Female
Humans
New Zealand
Pectins / metabolism*
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.415
|
| 引用数 |
5
|
| リソース情報 |
| 一般微生物 |
JCM 31914 |
