RRC ID 49317
著者 Yoshida T, Hamano A, Ueda A, Takeuchi H, Yamaoka S.
タイトル Human SMOOTHENED inhibits human immunodeficiency virus type 1 infection.
ジャーナル Biochem Biophys Res Commun
Abstract Human SMOOTHENED (SMO) was identified by expression cloning as a new host factor that inhibits HIV-1 infection. Forced expression of SMO inhibited HIV-1 replication and infection with a single-round lentiviral vector, but not infection with a murine leukemia virus-based retroviral vector in human MT-4 T cells. Quantitative PCR analyses revealed that stable expression of SMO impaired formation of the integrated form of lentiviral DNA, but did not interrupt reverse transcription. This inhibition was evident in MT-4 and HUT102 human T cell lines expressing low levels of SMO mRNA, but not in SupT1 or Jurkat T cell lines expressing higher levels of SMO mRNA. Depletion of SMO mRNA in Jurkat cells facilitated HIV-1 vector infection, suggesting that endogenous SMO plays a role in limiting lentiviral infection. These results suggest that SMO inhibits HIV-1 replication after completion of reverse transcription but before integration.
巻・号 493(1)
ページ 132-138
公開日 2017-11-4
DOI 10.1016/j.bbrc.2017.09.063
PII S0006-291X(17)31823-5
PMID 28917838
MeSH HIV Infections / immunology* HIV-1 / immunology* Humans Jurkat Cells Smoothened Receptor / immunology* Virus Internalization* Virus Replication / immunology*
IF 2.985
引用数 1
リソース情報
遺伝子材料 pCAG-HIVgp IN-D64V (RDB08256) CSII-CMV-MCS-IRES2-Bsd (RDB04385).