RRC ID 36326
著者 Chen Z, Nohata J, Guo H, Li S, Liu J, Guo Y, Yamamoto K, Kadono-Okuda K, Liu C, Arunkumar KP, Nagaraju J, Zhang Y, Liu S, Labropoulou V, Swevers L, Tsitoura P, Iatrou K, Gopinathan KP, Goldsmith MR, Xia Q, Mita K.
タイトル Construction, complete sequence, and annotation of a BAC contig covering the silkworm chorion locus.
ジャーナル Sci Data
Abstract The silkmoth chorion was studied extensively by F.C. Kafatos' group for almost 40 years. However, the complete structure of the chorion locus was not obtained in the genome sequence of Bombyx mori published in 2008 due to repetitive sequences, resulting in gaps and an incomplete view of the locus. To obtain the complete sequence of the chorion locus, expressed sequence tags (ESTs) derived from follicular epithelium cells were used as probes to screen a bacterial artificial chromosome (BAC) library. Seven BACs were selected to construct a contig which covered the whole chorion locus. By Sanger sequencing, we successfully obtained complete sequences of the chorion locus spanning 871,711 base pairs on chromosome 2, where we annotated 127 chorion genes. The dataset reported here will recruit more researchers to revisit one of the oldest model systems which has been used to study developmentally regulated gene expression. It also provides insights into egg development and fertilization mechanisms and is relevant to applications related to improvements in breeding procedures and transgenesis.
巻・号 2
ページ 150062
公開日 2015-1-1
DOI 10.1038/sdata.2015.62
PII sdata201562
PMID 26594380
PMC PMC4640134
MeSH Animals Bombyx / embryology Bombyx / genetics* Chorion* Chromosome Mapping Chromosome Structures Chromosomes, Artificial, Bacterial Expressed Sequence Tags Gene Library Genome, Insect* Molecular Sequence Annotation
IF 5.541
引用数 1
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