RRC ID 36339
著者 Yasukochi Y, Ohno M, Shibata F, Jouraku A, Nakano R, Ishikawa Y, Sahara K.
タイトル A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm.
ジャーナル Heredity (Edinb)
Abstract A significant feature of the genomes of Lepidoptera, butterflies and moths, is the high conservation of chromosome organization. Recent remarkable progress in genome sequencing of Lepidoptera has revealed that syntenic gene order is extensively conserved across phylogenetically distant species. The ancestral karyotype of Lepidoptera is thought to be n=31; however, that of the most well-studied moth, Bombyx mori, is n=28, and diverse studies suggest that three chromosomal fusion events occurred in this lineage. To identify the boundaries between predicted ancient fusions involving B. mori chromosomes 11, 23 and 24, we constructed fluorescence in situ hybridization (FISH)-based chromosome maps of the European corn borer, Ostrinia nubilalis (n=31). We first determined a 511 Mb genomic sequence of the Asian corn borer, O. furnacalis, a congener of O. nubilalis, and isolated bacterial artificial chromosomes and fosmid clones that were expected to localize in candidate regions for the boundaries using these sequences. Combined with FISH and genetic analysis, we narrowed down the candidate regions to 40 kb-1.5 Mb, in strong agreement with a previous estimate based on the genome of a butterfly, Melitaea cinxia. The significant difference in the lengths of the candidate regions where no functional genes were observed may reflect the evolutionary time after fusion events.
巻・号 116(1)
ページ 75-83
公開日 2016-1-1
DOI 10.1038/hdy.2015.72
PII hdy201572
PMID 26264548
PMC PMC4675876
MeSH Animals Biological Evolution* Chromosome Mapping* Chromosomes, Artificial, Bacterial Genes, Insect Genome, Insect* Genotype In Situ Hybridization, Fluorescence Male Moths / genetics* Synteny* Telomere / genetics Zea mays
IF 3.436
引用数 13
WOS 分野 GENETICS & HEREDITY ECOLOGY EVOLUTIONARY BIOLOGY
リソース情報
カイコ p50