RRC ID 51135
著者 Kobayashi H, Nakasato T, Sakamoto M, Ohtani Y, Terada F, Sakai K, Ohkuma M, Tohno M.
タイトル Clostridium pabulibutyricum sp. nov., a butyric-acid-producing organism isolated from high-moisture grass silage.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-stain-variable, strictly anaerobic, rod-shaped, catalase-negative and endospore-forming bacterial strain, designated MJC39T, was isolated from grass silage preserved in Hokkaido, Japan. Growth occurred at 20-42 °C, pH 5.0-7.0 and NaCl concentrations up to 2 % (w/v). The isolated strain MJC39T produced butyric acid in peptone yeast extract medium with glucose. The DNA G+C content of strain MJC39T was 34.4±0.2 mol%. The major cellular fatty acids (>10 %) were C14 : 0, C16 : 0 and summed feature 3 (including C16 : 1ω7c/C16 : 1ω6c). No respiratory quinones were detected. The polar lipids of strain MJC39T were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified lipid, one unidentified aminolipid, two unidentified glycolipids, one unidentified phospholipid, one unidentified aminoglycolipid and one unidentified phosphoaminoglycolipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJC39T was a member of the genus Clostridium and is closely related to Clostridium tyrobutyricum JCM 11008T (95.8 % similarity) and Clostridium algifaecis MB9-7T (95.5 % similarity). Based on the genotypic, phenotypic and chemotaxonomic characteristics, strain MJC39T represents a novel species of the genus Clostridium, for which the name Clostridium pabulibutyricum sp. nov. is proposed. The type strain is MJC39T (=JCM 31506T=DSM 103944T).
巻・号 67(12)
ページ 4974-4978
公開日 2017-12-1
DOI 10.1099/ijsem.0.002387
PMID 29034861
MeSH Bacterial Typing Techniques Base Composition Butyric Acid / metabolism* Clostridium / classification* Clostridium / genetics Clostridium / isolation & purification DNA, Bacterial / genetics Fatty Acids / chemistry Glycolipids / chemistry Japan Phospholipids / chemistry Phylogeny* Poaceae / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Silage / microbiology*
IF 2.415
引用数 5
リソース情報
一般微生物 JCM 31506 JCM 11008