RRC ID 49429
著者 Kondo Y, Minegishi H, Echigo A, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Tanaka A, Takahashi-Ando N, Fukushima Y, Yoshida Y, Ihara K, Usami R.
タイトル Haloparvum alkalitolerans sp. nov., alkali-tolerant haloarchaeon isolated from commercial salt.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-stain-negative, rod-pleomorphic, aerobic, halophilic archaeon, strain MK62-1T, was isolated from commercial salt made from seawater in the Philippines. Strain MK62-1T was able to grow at 2.1-4.7 M NaCl (with optimum at 2.1-2.6 M NaCl), pH 6.5-9.5 (optimum, pH 7.0-7.5) and 20-55 °C (optimum, 45-50 °C). Based on the orthologous 16S rRNA gene sequence, the closest relative was Haloparvum sedimenti JCM 30891T with 99.2 % similarity. The RNA polymerase subunit B' gene sequence also showed the highest similarity (97.4 %) to that of Haloparvum sedimenti DYS4T. The DNA G+C content of MK62-1T was 70.1 mol%, while that of Haloparvum sedimenti JCM 30891T was 69.5 mol% by the HPLC method. The levels of DNA-DNA relatedness between MK62-1T and Haloparvum sedimenti JCM 30891T were 60.6 and 60.8 % (reciprocally). The major polar lipids of the isolate were C20C20 archaeol derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Based on the phenotypic and phylogenetic analyses, it is proposed that the isolate represents species within the genus Haloparvum, for which the name Haloparvum alkalitolerans sp. nov. is proposed. The type strain is MK62-1T (=JCM 30442T =KCTC 4214T).
巻・号 66(12)
ページ 5314-5319
公開日 2016-12-1
DOI 10.1099/ijsem.0.001513
PMID 27666573
MeSH Alkalies Base Composition DNA, Archaeal / genetics Genes, Archaeal Halobacteriaceae / classification* Halobacteriaceae / genetics Halobacteriaceae / isolation & purification Nucleic Acid Hybridization Philippines Phospholipids / chemistry Phylogeny* RNA Polymerase II / genetics RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA Sodium Chloride*
IF 2.415
引用数 3
リソース情報
一般微生物 JCM 30442 JCM 30891