RRC ID 52661
著者 Tahara K, Kobayashi M, Yoshida S, Onodera R, Inoue N, Takeuchi H.
タイトル Effects of cationic liposomes with stearylamine against virus infection.
ジャーナル Int J Pharm
Abstract In this study, we demonstrated that cationic liposomes with incorporated stearylamine (SA) inhibit viral infectivity without preloaded active pharmaceutical ingredients. Specifically, we correlated physiochemical properties of liposomes, such as zeta potentials and particle sizes, with virus infectivity using the BacMam™ reagent, which is based on recombinant baculovirus (BV). Compared with neutral or negatively-charged liposomes, SA liposomes suppressed BV infectivity in several mammalian cell lines, including A549 cells. SA liposomes inhibited BV infection over 80% by optimizing the liposomal concentration and exposure time with cells. Moreover, these antiviral SA liposomes were not cytotoxic, and reducing the embedded cholesterol contents intensified the antiviral effects and simultaneously increased the binding of SA liposomes to the cell membranes. These data indicate that binding of SA liposomes to cell membranes may block virus entry. Finally, we also demonstrated the antiviral effects of SA liposomes on herpes simplex virus type 1 in A549 cells, and showed comparable efficacy to that of the antiviral drug acyclovir.
巻・号 543(1-2)
ページ 311-317
公開日 2018-5-30
DOI 10.1016/j.ijpharm.2018.04.001
PII S0378-5173(18)30212-6
PMID 29625169
MeSH A549 Cells Acyclovir / administration & dosage Acyclovir / chemistry Amines / administration & dosage* Amines / chemistry Animals Antiviral Agents / administration & dosage* Antiviral Agents / chemistry Baculoviridae / drug effects* Baculoviridae / physiology Cations Cell Survival / drug effects Chlorocebus aethiops Herpesvirus 1, Human / drug effects* Herpesvirus 1, Human / physiology Host-Pathogen Interactions Humans Liposomes Vero Cells Viral Plaque Assay Virus Diseases
IF 4.845
引用数 6
リソース情報
ヒト・動物細胞 A549(RCB0098) B16/BL6(RCB2638)