RRC ID 47930
著者 Kimura T, Takehana Y, Naruse K.
タイトル pnp4a Is the Causal Gene of the Medaka Iridophore Mutant guanineless.
ジャーナル G3 (Bethesda)
Abstract See-through medaka lines are suitable for observing internal organs throughout life. They were bred by crossing multiple color mutants. However, some of the causal genes for these mutants have not been identified. The medaka has four pigment cell types: black melanophores, yellow xanthophores, white leucophores, and silvery iridophores. The causal genes of melanophore, xanthophore, and leucophore mutants have been elucidated, but the causal gene for the iridophore mutant remains unknown. Here, we describe the iridophore mutant, guanineless (gu), which exhibits a strong reduction in visible iridophores throughout its larval to adult stages. The gu locus was previously mapped to chromosome 5, but was located near the telomeric region, making it difficult to integrate into the chromosome. We sought the causal gene of gu using synteny analysis with the zebrafish genome and found a strong candidate, purine nucleoside phosphorylase 4a (pnp4a). Gene targeting and complementation testing showed that pnp4a is the causal gene of gu This result will allow the establishment of inbred medaka strains or other useful strains with see-through phenotypes without major disruption in the genetic background of each strain.
巻・号 7(4)
ページ 1357-1363
公開日 2017-4-3
DOI 10.1534/g3.117.040675
PII g3.117.040675
PMID 28258112
PMC PMC5386883
MeSH Amino Acid Sequence Animals Base Sequence CRISPR-Cas Systems / genetics Exons / genetics Fish Proteins / chemistry Fish Proteins / genetics* Gene Expression Regulation, Developmental Gene Targeting Genetic Loci Heterozygote Melanophores / metabolism* Mutation / genetics* Oryzias / embryology Oryzias / genetics Oryzias / metabolism* Phenotype Phylogeny Pigmentation Synteny / genetics
IF 2.781
引用数 7
WOS 分野 GENETICS & HEREDITY
リソース情報
メダカ T5 (MT827) gu (MT58 ) d-rR/TOKYO (MT837) Full length cDNA (olec1e13)