| RRC ID |
51433
|
| 著者 |
Florica RO, Hipolito V, Bautista S, Anvari H, Rapp C, El-Rass S, Asgharian A, Antonescu CN, Killeen MT.
|
| タイトル |
The ENU-3 protein family members function in the Wnt pathway parallel to UNC-6/Netrin to promote motor neuron axon outgrowth in C. elegans.
|
| ジャーナル |
Dev Biol
|
| Abstract |
The axons of the DA and DB classes of motor neurons fail to reach the dorsal cord in the absence of the guidance cue UNC-6/Netrin or its receptor UNC-5 in C. elegans. However, the axonal processes usually exit their cell bodies in the ventral cord in the absence of both molecules. Strains lacking functional versions of UNC-6 or UNC-5 have a low level of DA and DB motor neuron axon outgrowth defects. We found that mutations in the genes for all six of the ENU-3 proteins function to enhance the outgrowth defects of the DA and DB axons in strains lacking either UNC-6 or UNC-5. A mutation in the gene for the MIG-14/Wntless protein also enhances defects in a strain lacking either UNC-5 or UNC-6, suggesting that the ENU-3 and Wnt pathways function parallel to the Netrin pathway in directing motor neuron axon outgrowth. Our evidence suggests that the ENU-3 proteins are novel members of the Wnt pathway in nematodes. Five of the six members of the ENU-3 family are predicted to be single-pass trans-membrane proteins. The expression pattern of ENU-3.1 was consistent with plasma membrane localization. One family member, ENU-3.6, lacks the predicted signal peptide and the membrane-spanning domain. In HeLa cells ENU-3.6 had a cytoplasmic localization and caused actin dependent processes to appear. We conclude that the ENU-3 family proteins function in a pathway parallel to the UNC-6/Netrin pathway for motor neuron axon outgrowth, most likely in the Wnt pathway.
|
| 巻・号 |
430(1)
|
| ページ |
249-261
|
| 公開日 |
2017-10-1
|
| DOI |
10.1016/j.ydbio.2017.06.036
|
| PII |
S0012-1606(17)30224-5
|
| PMID |
28694018
|
| MeSH |
Animals
Axons / metabolism*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism*
Cell Body / metabolism
Cell Nucleus / metabolism
Endoplasmic Reticulum / metabolism
Green Fluorescent Proteins / metabolism
HeLa Cells
Humans
Membrane Proteins / metabolism*
Motor Neurons / metabolism*
Nerve Tissue Proteins / metabolism*
Netrins
Neuronal Outgrowth*
Wnt Signaling Pathway*
|
| IF |
2.896
|
| 引用数 |
0
|
| リソース情報 |
| 線虫 |
tm3797
tm4519
tm5039
tm5189
tm5256
tm6465 |
