RRC ID 12646
著者 Teakle NL, Amtmann A, Real D, Colmer TD.
タイトル Lotus tenuis tolerates combined salinity and waterlogging: maintaining O2 transport to roots and expression of an NHX1-like gene contribute to regulation of Na+ transport.
ジャーナル Physiol Plant
Abstract Salinity and waterlogging interact to reduce growth for most crop and pasture species. The combination of these stresses often cause a large increase in the rate of Na(+) and Cl(-) transport to shoots; however, the mechanisms responsible for this are largely unknown. To identify mechanisms contributing to the adverse interaction between salinity and waterlogging, we compared two Lotus species with contrasting tolerances when grown under saline (200 mM NaCl) and O(2)-deficient (stagnant) treatments. Measurements of radial O(2) loss (ROL) under stagnant conditions indicated that more O(2) reaches root tips of Lotus tenuis, compared with Lotus corniculatus. Better internal aeration would contribute to maintaining Na(+) and Cl(-) transport processes in roots of L. tenuis exposed to stagnant-plus-NaCl treatments. L. tenuis root Na(+) concentrations after stagnant-plus-NaCl treatment (200 mM) were 17% higher than L. corniculatus, with 55% of the total plant Na(+) being accumulated in roots, compared with only 39% for L. corniculatus. L. tenuis accumulated more Na(+) in roots, presumably in vacuoles, thereby reducing transport to the shoot (25% lower than L. corniculatus). A candidate gene for vacuole Na(+) accumulation, an NHX1-like gene, was cloned from L. tenuis and identity established via sequencing and yeast complementation. Transcript levels of NHX1 in L. tenuis roots under stagnant-plus-NaCl treatment were the same as for aerated NaCl, whereas L. corniculatus roots had reduced transcript levels. Enhanced O(2) transport to roots enables regulation of Na(+) transport processes in L. tenuis roots, contributing to tolerance to combined salinity and waterlogging stresses.
巻・号 139(4)
ページ 358-74
公開日 2010-8-1
DOI 10.1111/j.1399-3054.2010.01373.x
PII PPL1373
PMID 20444189
MeSH Amino Acid Sequence Biological Transport Cation Transport Proteins / genetics Cation Transport Proteins / metabolism* Cloning, Molecular Gene Library Genes, Plant Lotus / genetics Lotus / physiology* Molecular Sequence Data Oxygen / metabolism* Plant Proteins / genetics Plant Proteins / metabolism* Plant Roots / metabolism Plant Roots / physiology RNA, Plant / genetics Salinity Sequence Alignment Sequence Analysis, DNA Sodium Chloride / pharmacology Sodium-Hydrogen Exchangers / genetics Sodium-Hydrogen Exchangers / metabolism* Stress, Physiological Water / physiology
IF 4.148
引用数 19
WOS 分野 PLANT SCIENCES
リソース情報
酵母 BY20135(W303-1B)