RRC ID |
19663
|
著者 |
Bak EJ, Ishii Y, Omatsu T, Kyuwa S, Tetsuya T, Hayasaka I, Yoshikawa Y.
|
タイトル |
Identification and analysis of MHC class II DRB1 (Patr-DRB1) alleles in chimpanzees.
|
ジャーナル |
Tissue Antigens
|
Abstract |
The MHC-DRB1 gene is known to display the most extensive allelic polymorphisms among MHC class II genes. We attempted the selective identification of chimpanzee (Pan troglodytes) DRB1 (Patr-DRB1) alleles using the polymerase chain reaction (PCR) technique in three steps: first, we performed Patr-DRB1*02 lineage-specific 8-kb PCR for *02 lineage detection in each chimpanzee; second, we performed 620-bp PCR for amplification of full-length exon 2; and finally, we carried out an insert check using the pattern of microsatellite repeat length variability. In the genomic DNA of 23 chimpanzees, nine Patr-DRB1 alleles containing two new alleles were detected. Our approach provides a relatively effective method of identifying Patr-DRB1 alleles in individual chimpanzees and should also contribute to our understanding of the features of MHC molecules in non-human primates.
|
巻・号 |
67(2)
|
ページ |
134-42
|
公開日 |
2006-2-1
|
DOI |
10.1111/j.1399-0039.2006.00539.x
|
PII |
TAN539
|
PMID |
16441484
|
MeSH |
Alleles*
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Genes, MHC Class II
Genetic Variation*
HLA-DR Antigens / genetics*
HLA-DRB1 Chains
Microsatellite Repeats
Molecular Sequence Data
Pan troglodytes / genetics*
Pan troglodytes / immunology
Polymorphism, Genetic*
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Species Specificity
|
IF |
1.348
|
引用数 |
5
|
WOS 分野
|
PATHOLOGY
IMMUNOLOGY
CELL BIOLOGY
|
リソース情報 |
GAIN |
|