RRC ID 19663
著者 Bak EJ, Ishii Y, Omatsu T, Kyuwa S, Tetsuya T, Hayasaka I, Yoshikawa Y.
タイトル Identification and analysis of MHC class II DRB1 (Patr-DRB1) alleles in chimpanzees.
ジャーナル Tissue Antigens
Abstract The MHC-DRB1 gene is known to display the most extensive allelic polymorphisms among MHC class II genes. We attempted the selective identification of chimpanzee (Pan troglodytes) DRB1 (Patr-DRB1) alleles using the polymerase chain reaction (PCR) technique in three steps: first, we performed Patr-DRB1*02 lineage-specific 8-kb PCR for *02 lineage detection in each chimpanzee; second, we performed 620-bp PCR for amplification of full-length exon 2; and finally, we carried out an insert check using the pattern of microsatellite repeat length variability. In the genomic DNA of 23 chimpanzees, nine Patr-DRB1 alleles containing two new alleles were detected. Our approach provides a relatively effective method of identifying Patr-DRB1 alleles in individual chimpanzees and should also contribute to our understanding of the features of MHC molecules in non-human primates.
巻・号 67(2)
ページ 134-42
公開日 2006-2-1
DOI 10.1111/j.1399-0039.2006.00539.x
PII TAN539
PMID 16441484
MeSH Alleles* Amino Acid Sequence Animals Base Sequence Cloning, Molecular Genes, MHC Class II Genetic Variation* HLA-DR Antigens / genetics* HLA-DRB1 Chains Microsatellite Repeats Molecular Sequence Data Pan troglodytes / genetics* Pan troglodytes / immunology Polymorphism, Genetic* Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Species Specificity
IF 1.348
引用数 5
WOS 分野 PATHOLOGY IMMUNOLOGY CELL BIOLOGY
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