RRC ID 33341
著者 Köhler D, Montandon C, Hause G, Majovsky P, Kessler F, Baginsky S, Agne B.
タイトル Characterization of chloroplast protein import without Tic56, a component of the 1-megadalton translocon at the inner envelope membrane of chloroplasts.
ジャーナル Plant Physiol
Abstract We report on the characterization of Tic56, a unique component of the recently identified 1-MD translocon at the inner envelope membrane of chloroplasts (TIC) in Arabidopsis (Arabidopsis thaliana) comprising Tic20, Tic100, and Tic214. We isolated Tic56 by copurification with Tandem Affinity Purification-tagged Toc159 in the absence of precursor protein, indicating spontaneous and translocation-independent formation of the translocon at the outer envelope membrane of chloroplasts (TOC) and TIC supercomplexes. Tic56 mutant plants have an albino phenotype and are unable to grow without an external carbon source. Using specific enrichment of protein amino termini, we analyzed the tic56-1 and plastid protein import2 (toc159) mutants to assess the in vivo import capacity of plastids in mutants of an outer and inner envelope component of the anticipated TOC-TIC supercomplex. Inboth mutants, we observed processing of several import substrates belonging to various pathways. Our results suggest that despite the severe developmental defects, protein import into Tic56-deficient plastids is functional to a considerable degree, indicating the existence of alternative translocases at the inner envelope membrane.
巻・号 167(3)
ページ 972-90
公開日 2015-3-1
DOI 10.1104/pp.114.255562
PII pp.114.255562
PMID 25588737
PMC PMC4348784
MeSH Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / metabolism* Blotting, Western Chloroplasts / metabolism Chloroplasts / ultrastructure* Intracellular Membranes / metabolism* Isotope Labeling Membrane Transport Proteins / chemistry Membrane Transport Proteins / metabolism* Molecular Weight Mutation / genetics Peptides / metabolism Plant Leaves / metabolism Plant Leaves / ultrastructure Protein Transport Substrate Specificity
IF 6.902
引用数 24
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda02782