RRC ID 15380
著者 Kennell JA, Cadigan KM, Shakhmantsir I, Waldron EJ.
タイトル The microRNA miR-8 is a positive regulator of pigmentation and eclosion in Drosophila.
ジャーナル Dev Dyn
Abstract BACKGROUND:MicroRNAs (miRNAs) are short, non-coding RNAs that post-transcriptionally silence gene expression by binding to target mRNAs. Previous studies have identified the miRNA miR-8 as a pleiotropic regulator of Drosophila development, controlling body size and neuronal survival by targeting multiple mRNAs. In this study we demonstrate that miR-8 is also required for proper spatial patterning of pigment on the adult abdominal cuticle in females but not males.
RESULTS:Female adult flies lacking miR-8 exhibit decreased pigmentation of the dorsal abdomen, with a pattern of pigmentation similar to wild type flies grown at higher temperatures. This pigmentation defect in miR-8 mutants is independent of the previously reported body size defect, and miR-8 acts directly in the developing cuticle to regulate pigmentation patterning. The decrease in pigmentation in miR-8 mutants was more pronounced in flies grown at higher temperatures. We also found that loss of miR-8 dramatically affected the ability to eclose at higher temperatures.
CONCLUSION:Loss of miR-8 increased the sensitivity of Drosophila to higher temperatures for both pigmentation patterning and the ability to eclose. Together, these data suggest that miR-8 acts as a buffer to stabilize gene expression patterns in the midst of environmental variation.
巻・号 241(1)
ページ 161-8
公開日 2012-1-1
DOI 10.1002/dvdy.23705
PMID 22174085
PMC PMC3243916
MeSH Animals Animals, Genetically Modified Drosophila / anatomy & histology* Drosophila / genetics* Drosophila / growth & development* Female Male MicroRNAs / genetics MicroRNAs / metabolism* Pigmentation / genetics* Transgenes
IF 3.275
引用数 34
WOS 分野 ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ NP5247(DGRC#104917)