論文 - 詳細
RRC ID | 15380 |
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著者 | Kennell JA, Cadigan KM, Shakhmantsir I, Waldron EJ. |
タイトル | The microRNA miR-8 is a positive regulator of pigmentation and eclosion in Drosophila. |
ジャーナル | Dev Dyn |
Abstract |
BACKGROUND:MicroRNAs (miRNAs) are short, non-coding RNAs that post-transcriptionally silence gene expression by binding to target mRNAs. Previous studies have identified the miRNA miR-8 as a pleiotropic regulator of Drosophila development, controlling body size and neuronal survival by targeting multiple mRNAs. In this study we demonstrate that miR-8 is also required for proper spatial patterning of pigment on the adult abdominal cuticle in females but not males. RESULTS:Female adult flies lacking miR-8 exhibit decreased pigmentation of the dorsal abdomen, with a pattern of pigmentation similar to wild type flies grown at higher temperatures. This pigmentation defect in miR-8 mutants is independent of the previously reported body size defect, and miR-8 acts directly in the developing cuticle to regulate pigmentation patterning. The decrease in pigmentation in miR-8 mutants was more pronounced in flies grown at higher temperatures. We also found that loss of miR-8 dramatically affected the ability to eclose at higher temperatures. CONCLUSION:Loss of miR-8 increased the sensitivity of Drosophila to higher temperatures for both pigmentation patterning and the ability to eclose. Together, these data suggest that miR-8 acts as a buffer to stabilize gene expression patterns in the midst of environmental variation. |
巻・号 | 241(1) |
ページ | 161-8 |
公開日 | 2012-1-1 |
DOI | 10.1002/dvdy.23705 |
PMID | 22174085 |
PMC | PMC3243916 |
MeSH | Animals Animals, Genetically Modified Drosophila / anatomy & histology* Drosophila / genetics* Drosophila / growth & development* Female Male MicroRNAs / genetics MicroRNAs / metabolism* Pigmentation / genetics* Transgenes |
IF | 3.275 |
引用数 | 34 |
WOS 分野 | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
リソース情報 | |
ショウジョウバエ | NP5247(DGRC#104917) |