RRC ID 40817
著者 Nakayama M, Shimatani K, Ozawa T, Shigemune N, Tomiyama D, Yui K, Katsuki M, Ikeda K, Nonaka A, Miyamoto T.
タイトル Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis.
ジャーナル Biosci Biotechnol Biochem
Abstract Catechins are a class of polyphenols and have high anti-bacterial activity against various microorganisms. Here, we report the mechanism for antibacterial activity of epigallocatechin gallate (EGCg) against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin-binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis.
巻・号 79(5)
ページ 845-54
公開日 2015-1-1
DOI 10.1080/09168451.2014.993356
PMID 25559894
MeSH Amino Acid Sequence Anti-Bacterial Agents / pharmacology* Bacillus subtilis / drug effects* Bacillus subtilis / metabolism Bacillus subtilis / physiology Bacterial Proteins / antagonists & inhibitors Bacterial Proteins / chemistry Bacterial Proteins / metabolism* Carrier Proteins / chemistry Carrier Proteins / metabolism Catechin / analogs & derivatives* Catechin / chemistry Catechin / metabolism Catechin / pharmacology Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Glucose / metabolism Lipoproteins / chemistry Lipoproteins / metabolism Molecular Docking Simulation Molecular Sequence Data Oxygen / metabolism Spores, Bacterial / drug effects
IF 1.516
引用数 32
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 1465