RRC ID 21129
著者 Verbitskiy D, Merwe JA, Zehrmann A, Härtel B, Takenaka M.
タイトル The E-class PPR protein MEF3 of Arabidopsis thaliana can also function in mitochondrial RNA editing with an additional DYW domain.
ジャーナル Plant Cell Physiol
Abstract In plants, RNA editing is observed in mitochondria and plastids, changing selected C nucleotides into Us in both organelles. We here identify the PPR (pentatricopeptide repeat) protein MEF3 (mitochondrial editing factor 3) of the E domain PPR subclass by genetic mapping of a variation between ecotypes Columbia (Col) and Landsberg erecta (Ler) in Arabidopsis thaliana to be required for a specific RNA editing event in mitochondria. The Ler variant of MEF3 differs from Col in two amino acids in repeats 9 and 10, which reduce RNA editing levels at site atp4-89 to about 50% in Ler. In a T-DNA insertion line, editing at this site is completely lost. In Vitis vinifera the gene most similar to MEF3 continues into a DYW extension beyond the common E domain. Complementation assays with various combinations of PPR and E domains from the vine and A. thaliana proteins show that the vine E region can substitute for the A. thaliana E region with or without the DYW domain. These findings suggest that the additional DYW domain does not disturb the MEF3 protein function in mitochondrial RNA editing in A. thaliana.
巻・号 53(2)
ページ 358-67
公開日 2012-2-1
DOI 10.1093/pcp/pcr182
PII pcr182
PMID 22186180
MeSH Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Chromosome Mapping DNA, Bacterial Ecotype Genetic Complementation Test Mitochondria / genetics Mitochondria / metabolism* Molecular Sequence Data Mutagenesis, Insertional Protein Interaction Domains and Motifs RNA Editing* RNA, Plant / metabolism*
IF 4.062
引用数 21
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 psh12413