RRC ID 45352
著者 Chibazakura T, Toriyabe Y, Fujii H, Takahashi K, Kawakami M, Kuwamura H, Haga H, Ogura S, Abe F, Nakajima M, Yoshikawa H, Tanaka T.
タイトル 5-Aminolevulinic acid enhances cell death under thermal stress in certain cancer cell lines.
ジャーナル Biosci Biotechnol Biochem
Abstract 5-aminolevulinic acid (5-ALA) is contained in all organisms and a starting substrate for heme biosynthesis. Since administration of 5-ALA specifically leads cancer cells to accumulate protoporphyrin IX (PpIX), a potent photosensitizer, we tested if 5-ALA also serves as a thermosensitizer. 5-ALA enhanced heat-induced cell death of cancer cell lines such as HepG2, Caco-2, and Kato III, but not other cancer cell lines including U2-OS and normal cell lines including WI-38. Those 5-ALA-sensitive cancer cells, but neither U2-OS nor WI-38, accumulated intracellular PpIX and exhibited an increased reactive oxygen species (ROS) generation under thermal stress with 5-ALA treatment. In addition, blocking the PpIX-exporting transporter ABCG2 in U2-OS and WI-38 cells enhanced their cell death under thermal stress with 5-ALA. Finally, a ROS scavenger compromised the cell death enhancement by 5-ALA. These suggest that 5-ALA can sensitize certain cancer cells, but not normal cells, to thermal stress via accumulation of PpIX and increase of ROS generation.
巻・号 79(3)
ページ 422-31
公開日 2015-1-1
DOI 10.1080/09168451.2014.975186
PMID 25346276
MeSH ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters / metabolism Aminolevulinic Acid / pharmacology* Biological Transport / drug effects Cell Death / drug effects Cell Line, Tumor Ferrochelatase / metabolism Gene Expression Regulation, Neoplastic / drug effects Heat-Shock Response / drug effects* Humans Intracellular Space / drug effects Intracellular Space / metabolism Iron-Binding Proteins / metabolism Neoplasm Proteins / metabolism Protoporphyrins / metabolism Reactive Oxygen Species / metabolism
IF 1.516
引用数 4
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MRC-5(RCB0218) WI-38(RCB0702)