| RRC ID |
30959
|
| 著者 |
Yamashino T, Yamawaki S, Hagui E, Ishida K, Ueoka-Nakanishi H, Nakamichi N, Mizuno T.
|
| タイトル |
Clock-controlled and FLOWERING LOCUS T (FT)-dependent photoperiodic pathway in Lotus japonicus II: characterization of a microRNA implicated in the control of flowering time.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Plant circadian clock generates rhythms with a period close to 24 h, and it controls a wide variety of physiological and developmental events, including the transition to reproductive growth (or flowering). During the last decade, significant research progress in Arabidopsis thaliana has been made in defining the molecular mechanism by which the circadian clock regulates flowering time in response to changes in photoperiod. In Lotus japonicus, we have found that LjFTa, which encodes a ortholog of the Arabidopsis FLOWERING LOCUS T (FT), plays an important role in the promotion of flowering, but it is not clear how the expression of LjFTa is regulated in L. japonicus. Based on current knowledge of photoperiodic control of flowering time in A. thaliana, here we examined whether a microRNA is involved in the activation of LjFTa in L. japonicus. Two putative L. japonicus genes that are responsible for the production of miR172 (designated LjmiR172a and LjmiR172b) were cloned. Overexpression of LjmiR172a/b in A. thaliana resulted in markedly accelerated flowering through enhancement of the expression of FT, concomitantly reducing the expression level of TARGET OF EARLY ACTIVATION TAGGED 1 (TOE1) transcripts, the protein product of which functions as a transcriptional repressor of FT. These results suggest that LjmiR172 genes play a positive role in the LjFTa-mediated promotion of flowering in L. japonicus.
|
| 巻・号 |
77(6)
|
| ページ |
1179-85
|
| 公開日 |
2013-1-1
|
| DOI |
10.1271/bbb.120872
|
| PII |
DN/JST.JSTAGE/bbb/120872
|
| PMID |
23748785
|
| MeSH |
Arabidopsis / genetics
Arabidopsis / growth & development
Arabidopsis Proteins / genetics
Circadian Clocks / genetics
Circadian Rhythm / genetics
DNA-Binding Proteins / genetics
Flowers / genetics
Flowers / growth & development*
Gene Expression Regulation, Plant
Lotus / genetics
Lotus / growth & development*
MicroRNAs / genetics*
MicroRNAs / metabolism
Photoperiod*
Sequence Homology, Amino Acid
|
| IF |
1.516
|
| 引用数 |
5
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ミヤコグサ・ダイズ |
Miyakojima MG-20 |
