RRC ID 36709
著者 Gillman JD, Stacey MG, Cui Y, Berg HR, Stacey G.
タイトル Deletions of the SACPD-C locus elevate seed stearic acid levels but also result in fatty acid and morphological alterations in nitrogen fixing nodules.
ジャーナル BMC Plant Biol
Abstract BACKGROUND:Soybean (Glycine max) seeds are the primary source of edible oil in the United States. Despite its widespread utility, soybean oil is oxidatively unstable. Until recently, the majority of soybean oil underwent chemical hydrogenation, a process which also generates trans fats. An alternative to chemical hydrogenation is genetic modification of seed oil through identification and introgression of mutant alleles. One target for improvement is the elevation of a saturated fat with no negative cardiovascular impacts, stearic acid, which typically constitutes a minute portion of seed oil (~3%).
RESULTS:We examined radiation induced soybean mutants with moderately increased stearic acid (10-15% of seed oil, ~3-5 X the levels in wild-type soybean seeds) via comparative whole genome hybridization and genetic analysis. The deletion of one SACPD isoform encoding gene (SACPD-C) was perfectly correlated with moderate elevation of seed stearic acid content. However, SACPD-C deletion lines were also found to have altered nodule fatty acid composition and grossly altered morphology. Despite these defects, overall nodule accumulation and nitrogen fixation were unaffected, at least under laboratory conditions.
CONCLUSIONS:Although no yield penalty has been reported for moderate elevated seed stearic acid content in soybean seeds, our results demonstrate that genetic alteration of seed traits can have unforeseen pleiotropic consequences. We have identified a role for fatty acid biosynthesis, and SACPD activity in particular, in the establishment and maintenance of symbiotic nitrogen fixation.
巻・号 14
ページ 143
公開日 2014-5-27
DOI 10.1186/1471-2229-14-143
PII 1471-2229-14-143
PMID 24886084
PMC PMC4058718
MeSH Amino Acid Sequence Chromosome Segregation Chromosomes, Plant / genetics Comparative Genomic Hybridization Crosses, Genetic Ethyl Methanesulfonate Fatty Acids / metabolism* Gene Deletion* Gene Expression Regulation, Plant Genes, Plant Genetic Loci Molecular Sequence Data Neutron Diffraction Nitrogen Fixation* Phenotype Plant Proteins / chemistry Plant Proteins / genetics* Plant Proteins / metabolism Root Nodules, Plant / anatomy & histology* Root Nodules, Plant / metabolism Seeds / metabolism* Sequence Analysis, DNA Soybean Oil Soybeans / genetics Stearic Acids / metabolism*
IF 3.497
引用数 22
WOS 分野 PLANT SCIENCES
リソース情報
ミヤコグサ・ダイズ KK24 MM106 M25