RRC ID 52694
著者 Iida S, Shimba K, Sakai K, Kotani K, Jimbo Y.
タイトル Synchronous firing patterns of induced pluripotent stem cell-derived cortical neurons depend on the network structure consisting of excitatory and inhibitory neurons.
ジャーナル Biochem Biophys Res Commun
Abstract The balance between glutamate-mediated excitation and GABA-mediated inhibition is critical to cortical functioning. However, the contribution of network structure consisting of the both neurons to cortical functioning has not been elucidated. We aimed to evaluate the relationship between the network structure and functional activity patterns in vitro. We used mouse induced pluripotent stem cells (iPSCs) to construct three types of neuronal populations; excitatory-rich (Exc), inhibitory-rich (Inh), and control (Cont). Then, we analyzed the activity patterns of these neuronal populations using microelectrode arrays (MEAs). Inhibitory synaptic densities differed between the three types of iPSC-derived neuronal populations, and the neurons showed spontaneously synchronized bursting activity with functional maturation for one month. Moreover, different firing patterns were observed between the three populations; Exc demonstrated the highest firing rates, including frequent, long, and dominant bursts. In contrast, Inh demonstrated the lowest firing rates and the least dominant bursts. Synchronized bursts were enhanced by disinhibition via GABAA receptor blockade. The present study, using iPSC-derived neurons and MEAs, for the first time show that synchronized bursting of cortical networks in vitro depends on the network structure consisting of excitatory and inhibitory neurons.
巻・号 501(1)
ページ 152-157
公開日 2018-6-18
DOI 10.1016/j.bbrc.2018.04.197
PII S0006-291X(18)30994-X
PMID 29723524
MeSH Action Potentials Animals Bicuculline / analogs & derivatives Bicuculline / pharmacology Cell Differentiation Cell Line Cerebral Cortex / cytology* Cerebral Cortex / physiology* GABA-A Receptor Antagonists / pharmacology GABAergic Neurons / cytology GABAergic Neurons / drug effects GABAergic Neurons / physiology Glutamic Acid / physiology Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / physiology* Mice Microelectrodes Nerve Net / cytology Nerve Net / physiology Neurons / cytology Neurons / drug effects Neurons / physiology* Receptors, GABA-A / physiology
IF 2.985
引用数 6
リソース情報
ヒト・動物細胞 iPS-Stm-FB/gfp-99-1(APS0005)