RRC ID 47770
著者 Ueta R, Abe C, Watanabe T, Sugano SS, Ishihara R, Ezura H, Osakabe Y, Osakabe K.
タイトル Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9.
ジャーナル Sci Rep
Abstract Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9-a key gene controlling parthenocarpy-with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit-a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.
巻・号 7(1)
ページ 507
公開日 2017-3-30
DOI 10.1038/s41598-017-00501-4
PII 10.1038/s41598-017-00501-4
PMID 28360425
PMC PMC5428692
MeSH Base Sequence Breeding* CRISPR-Cas Systems / genetics* Gene Knockout Techniques Mutagenesis, Site-Directed Mutation / genetics Parthenogenesis / genetics* Phenotype Plants, Genetically Modified RNA, Guide, Kinetoplastida / genetics Solanum lycopersicum / genetics* Solanum lycopersicum / growth & development*
IF 3.998
引用数 67
WOS 分野 PLANT SCIENCES
リソース情報
トマト TOMJPF00001