RRC ID 50302
著者 Gálisová A, Fábryová E, Sticová E, Kosinová L, Jirátová M, Herynek V, Berková Z, Kříž J, Hájek M, Jirák D.
タイトル The Optimal Timing for Pancreatic Islet Transplantation into Subcutaneous Scaffolds Assessed by Multimodal Imaging.
ジャーナル Contrast Media Mol Imaging
Abstract Subcutaneously implanted polymeric scaffolds represent an alternative transplantation site for pancreatic islets (PIs) with the option of vascularisation enhancement by mesenchymal stem cells (MSC). Nevertheless, a proper timing of the transplantation steps is crucial. In this study, scaffolds supplemented with plastic rods were implanted into diabetic rats and two timing schemes for subsequent transplantation of bioluminescent PIs (4 or 7 days after rod removal) were examined by multimodal imaging. The cavities were left to heal spontaneously or with 10 million injected MSCs. Morphological and vascularisation changes were examined by MRI, while the localisation and viability of transplanted islets were monitored by bioluminescence imaging. The results show that PIs transplanted 4 days after rod removal showed the higher optical signal and vascularisation compared to transplantation after 7 days. MSCs slightly improved vascularisation of the graft but hindered therapeutic efficiency of PIs. Long-term glycaemia normalisation (4 months) was attained in 80% of animals. In summary, multimodal imaging confirmed the long-term survival and function of transplanted PIs in the devices. The best outcome was reached with PIs transplanted on day 4 after rod removal and therefore the suggested protocol holds a potential for further applications.
巻・号 2017
ページ 5418495
公開日 2017-12-26
DOI 10.1155/2017/5418495
PMID 29440984
PMC PMC5758856
MeSH Allografts Animals Diabetes Mellitus, Experimental* / diagnostic imaging Diabetes Mellitus, Experimental* / surgery Graft Survival* Islets of Langerhans* / blood supply Islets of Langerhans* / diagnostic imaging Islets of Langerhans* / metabolism Islets of Langerhans* / pathology Islets of Langerhans Transplantation / methods* Luminescent Measurements* Male Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells / metabolism* Mesenchymal Stem Cells / pathology Rats Rats, Transgenic Tissue Scaffolds*
IF 2.934
リソース情報
ラット LEW-Tg(Gt(ROSA)26Sor-luc)11Jmsk (strainID=649)