RRC ID 38911
著者 Ichijo Y, Mochimaru Y, Azuma M, Satou K, Negishi J, Nakakura T, Oshima N, Mogi C, Sato K, Matsuda K, Okajima F, Tomura H.
タイトル Two zebrafish G2A homologs activate multiple intracellular signaling pathways in acidic environment.
ジャーナル Biochem Biophys Res Commun
Abstract Human G2A is activated by various stimuli such as lysophosphatidylcholine (LPC), 9-hydroxyoctadecadienoic acid (9-HODE), and protons. The receptor is coupled to multiple intracellular signaling pathways, including the Gs-protein/cAMP/CRE, G12/13-protein/Rho/SRE, and Gq-protein/phospholipase C/NFAT pathways. In the present study, we examined whether zebrafish G2A homologs (zG2A-a and zG2A-b) could respond to these stimuli and activate multiple intracellular signaling pathways. We also examined whether histidine residue and basic amino acid residue in the N-terminus of the homologs also play roles similar to those played by human G2A residues if the homologs sense protons. We found that the zG2A-a showed the high CRE, SRE, and NFAT activities, however, zG2A-b showed only the high SRE activity under a pH of 8.0. Extracellular acidification from pH 7.4 to 6.3 ameliorated these activities in zG2A-a-expressing cells. On the other hand, acidification ameliorated the SRE activity but not the CRE and NFAT activities in zG2A-b-expressing cells. LPC or 9-HODE did not modify any activity of either homolog. The substitution of histidine residue at the 174(th) position from the N-terminus of zG2A-a to asparagine residue attenuated proton-induced CRE and NFAT activities but not SRE activity. The substitution of arginine residue at the 32nd position from the N-terminus of zG2A-a to the alanine residue also attenuated its high and the proton-induced CRE and NFAT activities. On the contrary, the substitution did not attenuate SRE activity. The substitution of the arginine residue at the 10th position from the N-terminus of zG2A-b to the alanine residue also did not attenuate its high or the proton-induced SRE activity. These results indicate that zebrafish G2A homologs were activated by protons but not by LPC and 9-HODE, and the activation mechanisms of the homologs were similar to those of human G2A.
巻・号 469(1)
ページ 81-86
公開日 2016-1-1
DOI 10.1016/j.bbrc.2015.11.075
PII S0006-291X(15)30944-X
PMID 26614909
MeSH Amino Acid Sequence Animals Cell Cycle Proteins / chemistry* Cell Cycle Proteins / metabolism* HEK293 Cells Humans Hydrogen-Ion Concentration* Intracellular Fluid / chemistry Intracellular Fluid / metabolism Molecular Sequence Data Protein Binding Receptors, G-Protein-Coupled / chemistry* Receptors, G-Protein-Coupled / metabolism* Signal Transduction / physiology* Structure-Activity Relationship Zebrafish / metabolism*
IF 2.985
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
リソース情報
ヒト・動物細胞 293T(RCB2202)