RRC ID |
28109
|
著者 |
Singh P, Kuo YC, Mishra S, Tsai CH, Chien CC, Chen CW, Desclos-Theveniau M, Chu PW, Schulze B, Chinchilla D, Boller T, Zimmerli L.
|
タイトル |
The lectin receptor kinase-VI.2 is required for priming and positively regulates Arabidopsis pattern-triggered immunity.
|
ジャーナル |
Plant Cell
|
Abstract |
Plant cells can be sensitized toward a subsequent pathogen attack by avirulent pathogens or by chemicals such as β-aminobutyric acid (BABA). This process is called priming. Using a reverse genetic approach in Arabidopsis thaliana, we demonstrate that the BABA-responsive L-type lectin receptor kinase-VI.2 (LecRK-VI.2) contributes to disease resistance against the hemibiotrophic Pseudomonas syringae and the necrotrophic Pectobacterium carotovorum bacteria. Accordingly, LecRK-VI.2 mRNA levels increased after bacterial inoculation or treatments with microbe-associated molecular patterns (MAMPs). We also show that LecRK-VI.2 is required for full activation of pattern-triggered immunity (PTI); notably, lecrk-VI.2-1 mutants show reduced upregulation of PTI marker genes, impaired callose deposition, and defective stomatal closure. Overexpression studies combined with genome-wide microarray analyses indicate that LecRK-VI.2 positively regulates the PTI response. LecRK-VI.2 is demonstrated to act upstream of mitogen-activated protein kinase signaling, but independently of reactive oxygen production and Botrytis-induced kinase1 phosphorylation. In addition, complex formation between the MAMP receptor flagellin sensing2 and its signaling partner brassinosteroid insensitive1-associated kinase1 is observed in flg22-treated lecrk-VI.2-1 mutants. LecRK-VI.2 is also required for full BABA-induced resistance and priming of PTI. Our work identifies LecRK-VI.2 as a novel mediator of the Arabidopsis PTI response and provides insight into molecular mechanisms governing priming.
|
巻・号 |
24(3)
|
ページ |
1256-70
|
公開日 |
2012-3-1
|
DOI |
10.1105/tpc.112.095778
|
PII |
tpc.112.095778
|
PMID |
22427336
|
PMC |
PMC3336125
|
MeSH |
Aminobutyrates / pharmacology
Arabidopsis / enzymology
Arabidopsis / genetics*
Arabidopsis / immunology
Arabidopsis Proteins / genetics
Arabidopsis Proteins / immunology*
Arabidopsis Proteins / metabolism
DNA, Bacterial / genetics
Disease Resistance
Gene Expression Profiling
Gene Expression Regulation, Plant
Genetic Complementation Test
Mutagenesis, Insertional
Oligonucleotide Array Sequence Analysis
Pectobacterium carotovorum / pathogenicity
Plant Diseases / genetics
Plant Diseases / immunology
Plant Diseases / microbiology
Plant Immunity*
Plant Stomata / immunology
Protein Kinases / metabolism
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / immunology*
Protein Serine-Threonine Kinases / metabolism
Pseudomonas syringae / pathogenicity
RNA, Plant / genetics
|
IF |
9.618
|
引用数 |
119
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda01628 |