RRC ID 28109
著者 Singh P, Kuo YC, Mishra S, Tsai CH, Chien CC, Chen CW, Desclos-Theveniau M, Chu PW, Schulze B, Chinchilla D, Boller T, Zimmerli L.
タイトル The lectin receptor kinase-VI.2 is required for priming and positively regulates Arabidopsis pattern-triggered immunity.
ジャーナル Plant Cell
Abstract Plant cells can be sensitized toward a subsequent pathogen attack by avirulent pathogens or by chemicals such as β-aminobutyric acid (BABA). This process is called priming. Using a reverse genetic approach in Arabidopsis thaliana, we demonstrate that the BABA-responsive L-type lectin receptor kinase-VI.2 (LecRK-VI.2) contributes to disease resistance against the hemibiotrophic Pseudomonas syringae and the necrotrophic Pectobacterium carotovorum bacteria. Accordingly, LecRK-VI.2 mRNA levels increased after bacterial inoculation or treatments with microbe-associated molecular patterns (MAMPs). We also show that LecRK-VI.2 is required for full activation of pattern-triggered immunity (PTI); notably, lecrk-VI.2-1 mutants show reduced upregulation of PTI marker genes, impaired callose deposition, and defective stomatal closure. Overexpression studies combined with genome-wide microarray analyses indicate that LecRK-VI.2 positively regulates the PTI response. LecRK-VI.2 is demonstrated to act upstream of mitogen-activated protein kinase signaling, but independently of reactive oxygen production and Botrytis-induced kinase1 phosphorylation. In addition, complex formation between the MAMP receptor flagellin sensing2 and its signaling partner brassinosteroid insensitive1-associated kinase1 is observed in flg22-treated lecrk-VI.2-1 mutants. LecRK-VI.2 is also required for full BABA-induced resistance and priming of PTI. Our work identifies LecRK-VI.2 as a novel mediator of the Arabidopsis PTI response and provides insight into molecular mechanisms governing priming.
巻・号 24(3)
ページ 1256-70
公開日 2012-3-1
DOI 10.1105/tpc.112.095778
PII tpc.112.095778
PMID 22427336
PMC PMC3336125
MeSH Aminobutyrates / pharmacology Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis / immunology Arabidopsis Proteins / genetics Arabidopsis Proteins / immunology* Arabidopsis Proteins / metabolism DNA, Bacterial / genetics Disease Resistance Gene Expression Profiling Gene Expression Regulation, Plant Genetic Complementation Test Mutagenesis, Insertional Oligonucleotide Array Sequence Analysis Pectobacterium carotovorum / pathogenicity Plant Diseases / genetics Plant Diseases / immunology Plant Diseases / microbiology Plant Immunity* Plant Stomata / immunology Protein Kinases / metabolism Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / immunology* Protein Serine-Threonine Kinases / metabolism Pseudomonas syringae / pathogenicity RNA, Plant / genetics
IF 9.618
引用数 119
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda01628