RRC ID 34870
著者 Clemente P, Pajak A, Laine I, Wibom R, Wedell A, Freyer C, Wredenberg A.
タイトル SUV3 helicase is required for correct processing of mitochondrial transcripts.
ジャーナル Nucleic Acids Res
Abstract Mitochondrial gene expression is largely regulated by post-transcriptional mechanisms that control the amount and translation of each mitochondrial mRNA. Despite its importance for mitochondrial function, the mechanisms and proteins involved in mRNA turnover are still not fully characterized. Studies in yeast and human cell lines have indicated that the mitochondrial helicase SUV3, together with the polynucleotide phosphorylase, PNPase, composes the mitochondrial degradosome. To further investigate the in vivo function of SUV3 we disrupted the homolog of SUV3 in Drosophila melanogaster (Dm). Loss of dmsuv3 led to the accumulation of mitochondrial mRNAs, without increasing rRNA levels, de novo transcription or decay intermediates. Furthermore, we observed a severe decrease in mitochondrial tRNAs accompanied by an accumulation of unprocessed precursor transcripts. These processing defects lead to reduced mitochondrial translation and a severe respiratory chain complex deficiency, resulting in a pupal lethal phenotype. In summary, our results propose that SUV3 is predominantly required for the processing of mitochondrial polycistronic transcripts in metazoan and that this function is independent of PNPase.
巻・号 43(15)
ページ 7398-413
公開日 2015-9-3
DOI 10.1093/nar/gkv692
PII gkv692
PMID 26152302
PMC PMC4551930
MeSH Animals Cell Line DEAD-box RNA Helicases / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila Proteins / physiology* Drosophila melanogaster / genetics Electron Transport Genes, Lethal HeLa Cells Humans Mitochondria / genetics Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Mitochondrial Proteins / physiology* Polyribonucleotide Nucleotidyltransferase / genetics Protein Biosynthesis RNA / metabolism* RNA Helicases / genetics RNA Helicases / metabolism RNA Helicases / physiology* RNA Processing, Post-Transcriptional* RNA Stability RNA, Messenger / metabolism RNA, Mitochondrial RNA, Ribosomal / metabolism RNA, Transfer / metabolism
IF 11.502
引用数 12
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ 9791R-2