RRC ID 45181
著者 Gamo K, Miyachi H, Nakamura K, Matsuura N.
タイトル Hesperetin glucuronides induce adipocyte differentiation via activation and expression of peroxisome proliferator-activated receptor-γ.
ジャーナル Biosci Biotechnol Biochem
Abstract In previous reports, hesperidin, a flavonoid glucoside from citrus fruit, is hydrolyzed to hesperetin, an aglycone of hesperidin, and converted to the hesperetin glucuronides (H7-OG and H3'-OG) in vivo and depresses blood glucose levels. But there are no reports on the activity of hesperetin glucuronides. To determine the activity of hesperetin glucuronides, H7-OG and H3'-OG were synthesized and peroxisome proliferator-activated receptor-γ (PPARγ) agonist activity was observed at 250 μM. These glucuronides accelerated the differentiation of 3T3-L1 cells into adipocytes at 10 μM. Furthermore, H7-OG showed additive effects in reporter gene assays and caused noncompetitive reactions in time-resolved fluorescence resonance energy transfer assays with a thiazolidinedione derivative. Our results indicated that hesperetin glucuronides activated PPARγ, accelerated adipocyte differentiation.
巻・号 78(6)
ページ 1052-9
公開日 2014-1-1
DOI 10.1080/09168451.2014.910097
PMID 25036134
MeSH 3T3-L1 Cells Adipocytes / cytology* Adipocytes / drug effects Animals COS Cells Cell Differentiation / drug effects* Chlorocebus aethiops Chromans / pharmacology Drug Synergism Gene Expression Regulation / drug effects* Glucuronides / chemistry* Hesperidin / chemistry* Hesperidin / metabolism Hesperidin / pharmacology* Mice PPAR gamma / agonists PPAR gamma / chemistry PPAR gamma / genetics PPAR gamma / metabolism* Protein Structure, Tertiary Thiazolidinediones / pharmacology Troglitazone
IF 1.516
引用数 6
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 COS-1(RCB0143)