RRC ID 45529
著者 Bhatla N, Droste R, Sando SR, Huang A, Horvitz HR.
タイトル Distinct Neural Circuits Control Rhythm Inhibition and Spitting by the Myogenic Pharynx of C. elegans.
ジャーナル Curr Biol
Abstract Neural circuits have long been known to modulate myogenic muscles such as the heart, yet a mechanistic understanding at the cellular and molecular levels remains limited. We studied how light inhibits pumping of the Caenorhabditis elegans pharynx, a myogenic muscular pump for feeding, and found three neural circuits that alter pumping. First, light inhibits pumping via the I2 neuron monosynaptic circuit. Our electron microscopic reconstruction of the anterior pharynx revealed evidence for synapses from I2 onto muscle that were missing from the published connectome, and we show that these "missed synapses" are likely functional. Second, light inhibits pumping through the RIP-I1-MC neuron polysynaptic circuit, in which an inhibitory signal is likely transmitted from outside the pharynx into the pharynx in a manner analogous to how the mammalian autonomic nervous system controls the heart. Third, light causes a novel pharyngeal behavior, reversal of flow or "spitting," which is induced by the M1 neuron. These three neural circuits show that neurons can control a myogenic muscle organ not only by changing the contraction rate but also by altering the functional consequences of the contraction itself, transforming swallowing into spitting. Our observations also illustrate why connectome builders and users should be cognizant that functional synaptic connections might exist despite the absence of a declared synapse in the connectome.
巻・号 25(16)
ページ 2075-89
公開日 2015-8-17
DOI 10.1016/j.cub.2015.06.052
PII S0960-9822(15)00746-0
PMID 26212880
PMC PMC4546535
MeSH Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans / ultrastructure Feeding Behavior Microscopy, Electron, Transmission Motor Neurons / physiology Motor Neurons / ultrastructure Muscles / physiology Muscles / ultrastructure Pharynx / physiology Pharynx / ultrastructure Synapses / physiology Synapses / ultrastructure
IF 9.601
引用数 21
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm3390 tm3219 tm3506 tm2827 tm1811 tm355 tm1766 tm3239 tm2877 tm3990