RRC ID 47431
著者 Ito H, Sato K, Kondo S, Ueda R, Yamamoto D.
タイトル Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila.
ジャーナル Curr Biol
Abstract The Drosophila fruitless (fru) gene is regarded as a master regulator of the formation of male courtship circuitry, yet little is known about its molecular basis of action. We show that roundabout 1 (robo1) knockdown in females promotes formation of the male-specific neurite in sexually dimorphic mAL interneurons and that overexpression of the male-specific Fru(BM) diminishes the expression of Robo1 in the fly brain. Our electrophoretic mobility shift and reporter assays identify the 42-bp segment encompassing the palindrome sequence T T C G C T G C G C C G T G A A in the 5' UTR of robo1 exon1 as the Fru(BM)-responsive element. We find that ∼10-bp deletions in the palindrome sequence induce a loss of the male-specific neurite and disrupt male courtship patterns. This study paves the way for a thorough understanding of the mechanism whereby Fru proteins orchestrate transcription for the formation of courtship circuitry.
巻・号 26(12)
ページ 1532-1542
公開日 2016-6-20
DOI 10.1016/j.cub.2016.04.067
PII S0960-9822(16)30461-4
PMID 27265393
MeSH Animals Drosophila / growth & development Drosophila / metabolism* Drosophila Proteins / metabolism* Female Male Nerve Tissue Proteins / metabolism* Nervous System / growth & development* Receptors, Immunologic / metabolism* Sex Characteristics* Transcription Factors / metabolism*
IF 9.601
引用数 20
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ