RRC ID 48198
著者 Saiki A, Ishida Y, Segawa S, Hirota R, Nakamura T, Kuroda A.
タイトル A Lactobacillus mutant capable of accumulating long-chain polyphosphates that enhance intestinal barrier function.
ジャーナル Biosci Biotechnol Biochem
Abstract Inorganic polyphosphate (polyP) was previously identified as a probiotic-derived substance that enhances intestinal barrier function. PolyP-accumulating bacteria are expected to have beneficial effects on the human gastrointestinal tract. In this study, we selected Lactobacillus paracasei JCM 1163 as a strain with the potential to accumulate polyP, because among the probiotic bacteria stored in our laboratory, it had the largest amount of polyP. The chain length of polyP accumulated in L. paracasei JCM 1163 was approximately 700 phosphate (Pi) residues. L. paracasei JCM 1163 accumulated polyP when Pi was added to Pi-starved cells. We further improved the ability of L. paracasei JCM 1163 to accumulate polyP by nitrosoguanidine mutagenesis. The mutant accumulated polyP at a level of 1500 nmol/mg protein-approximately 190 times that of the wild-type strain. PolyP extracted from the L. paracasei JCM 1163 significantly suppressed the oxidant-induced intestinal permeability in mouse small intestine. In conclusion, we have succeeded in breeding the polyP-accumulating Lactobacillus mutant that is expected to enhance intestinal barrier function.
巻・号 80(5)
ページ 955-61
公開日 2016-5-1
DOI 10.1080/09168451.2015.1135041
PMID 26966939
MeSH Ammonium Chloride / antagonists & inhibitors Ammonium Chloride / pharmacology Animals Biological Transport / drug effects Intestine, Small / drug effects* Intestine, Small / metabolism Lacticaseibacillus paracasei / drug effects Lacticaseibacillus paracasei / genetics* Lacticaseibacillus paracasei / metabolism Male Mannitol / metabolism Mice Mice, Inbred C57BL Mutagenesis* Mutagens / pharmacology Nitrosoguanidines / pharmacology Oxidants / antagonists & inhibitors Oxidants / pharmacology Permeability / drug effects Polyphosphates / metabolism Polyphosphates / pharmacology* Probiotics / metabolism Probiotics / pharmacology* Selection, Genetic Tissue Culture Techniques
IF 1.516
引用数 4
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED
リソース情報
一般微生物 JCM 1163