RRC ID 49531
著者 Fujii Y, Maekawa S, Morita M.
タイトル Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels.
ジャーナル Sci Rep
Abstract Wave-like propagation of [Ca2+]i increases is a remarkable intercellular communication characteristic in astrocyte networks, intercalating neural circuits and vasculature. Mechanically-induced [Ca2+]i increases and their subsequent propagation to neighboring astrocytes in culture is a classical model of astrocyte calcium wave and is known to be mediated by gap junction and extracellular ATP, but the role of each pathway remains unclear. Pharmacologic analysis of time-dependent distribution of [Ca2+]i revealed three distinct [Ca2+]i increases, the largest being in stimulated cells independent of extracellular Ca2+ and inositol 1,4,5-trisphosphate-induced Ca2+ release. In addition, persistent [Ca2+]i increases were found to propagate rapidly via gap junctions in the proximal region, and transient [Ca2+]i increases were found to propagate slowly via extracellular ATP in the distal region. Simultaneous imaging of astrocyte [Ca2+]i and extracellular ATP, the latter of which was measured by an ATP sniffing cell, revealed that ATP was released within the proximal region by volume-regulated anion channel in a [Ca2+]i independent manner. This detailed analysis of a classical model is the first to address the different contributions of two major pathways of calcium waves, gap junctions and extracellular ATP.
巻・号 7(1)
ページ 13115
公開日 2017-10-13
DOI 10.1038/s41598-017-13243-0
PII 10.1038/s41598-017-13243-0
PMID 29030562
PMC PMC5640625
MeSH Adenosine Triphosphate / metabolism* Animals Astrocytes / metabolism* Fluorescent Antibody Technique Gap Junctions / metabolism* Ion Channels / metabolism* Lipopolysaccharides / metabolism* Male Rats Receptors, Lipoprotein / metabolism
IF 3.998
引用数 25
リソース情報
遺伝子材料 pN1-G-CaMP2 (RDB06750).