RRC ID 37123
著者 Hattori T, Nakashima K, Marutani T, Kiso Y, Nishi Y, Mukai H.
タイトル Successful acquisition of a neutralizing monoclonal antibody against a novel neutrophil-activating peptide, mitocryptide-1.
ジャーナル Biochem Biophys Res Commun
Abstract Mitocryptide-1 (MCT-1) is a novel neutrophil-activating peptide derived from mitochondrial cytochrome c oxidase subunit VIII, and its physiological role and involvement in various diseases have not yet been elucidated. Generating neutralizing antibodies against the function of MCT-1 is of particular importance for investigating its physiological and pathophysiological roles, because MCT-1 is a fragmented peptide of its mother protein and hence it is very difficult to manipulate its expression level genetically without affecting expression of the mother protein. Here, we report the successful generation of a neutralizing monoclonal antibody (MAb) against MCT-1. This MAb, designated NM1B1, which specifically bound to the region of positions 9-22 of MCT-1, showed concentration-dependent inhibition of MCT-1-induced migration and β-hexosaminidase release in neutrophilic/granulocytic differentiated HL-60 cells. Thus, NM1B1, as a neutralizing MAb against MCT-1, could elucidate not just the physiological regulatory mechanisms of MCT-1 but also its pathophysiological involvement in various inflammatory diseases in vivo.
巻・号 463(1-2)
ページ 54-9
公開日 2015-7-17
DOI 10.1016/j.bbrc.2015.05.016
PII S0006-291X(15)00922-5
PMID 25986736
MeSH Amino Acid Sequence Animals Antibodies, Monoclonal / immunology* Antibodies, Neutralizing / immunology* Antibody Specificity Electron Transport Complex IV / chemistry Electron Transport Complex IV / genetics Electron Transport Complex IV / immunology* Epitopes / chemistry Epitopes / genetics Female HL-60 Cells Humans Mice Mice, Inbred BALB C Molecular Sequence Data Neutrophils / immunology* Peptide Fragments / chemistry Peptide Fragments / genetics Peptide Fragments / immunology Protein Subunits / chemistry Protein Subunits / genetics Protein Subunits / immunology
IF 2.985
引用数 3
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HL-60(RCB3683)