RRC ID 50337
著者 Pulungan SI, Yano R, Okabe Y, Ichino T, Kojima M, Takebayashi Y, Sakakibara H, Ariizumi T, Ezura H.
タイトル SlLAX1 is Required for Normal Leaf Development Mediated by Balanced Adaxial and Abaxial Pavement Cell Growth in Tomato.
ジャーナル Plant Cell Physiol
Abstract Leaves are the major plant organs with a primary function for photosynthesis. Auxin controls various aspects of plant growth and development, including leaf initiation, expansion and differentiation. Unique and intriguing auxin features include its polar transport, which is mainly controlled by the AUX1/LAX and PIN gene families as influx and efflux carriers, respectively. The role of AUX1/LAX genes in root development is well documented, but the role of these genes in leaf morphogenesis remains unclear. Moreover, most studies have been conducted in the plant model Arabidopsis thaliana, while studies in tomato are still scarce. In this study, we isolated six lines of the allelic curly leaf phenotype 'curl' mutants from a γ-ray and EMS (ethyl methanesulfonate) mutagenized population. Using a map-based cloning strategy combined with exome sequencing, we observed that a mutation occurred in the SlLAX1 gene (Solyc09g014380), which is homologous to an Arabidopsis auxin influx carrier gene, AUX1 (AtAUX1). Characterization of six alleles of single curl mutants revealed the pivotal role of SlLAX1 in controlling tomato leaf flatness by balancing adaxial and abaxial pavement cell growth, which has not been reported in tomato. Using TILLING (Targeting Induced Local Lesions IN Genome) technology, we isolated an additional mutant allele of the SlLAX1 gene and this mutant showed a curled leaf phenotype similar to other curl mutants, suggesting that Solyc09g014380 is responsible for the curl phenotype. These results showed that SlLAX1 is required for normal leaf development mediated by balanced adaxial and abaxial pavement cell growth in tomato.
巻・号 59(6)
ページ 1170-1186
公開日 2018-3-1
DOI 10.1093/pcp/pcy052
PII 4924842
PMID 29528453
MeSH Alleles Amino Acid Sequence Chromosome Mapping Indoleacetic Acids / metabolism* Mutation Phenotype Plant Growth Regulators / metabolism* Plant Leaves / anatomy & histology Plant Leaves / genetics* Plant Leaves / growth & development Plant Proteins / genetics Plant Proteins / metabolism* Sequence Alignment Solanum lycopersicum / anatomy & histology Solanum lycopersicum / genetics* Solanum lycopersicum / growth & development
IF 4.062
引用数 5
リソース情報
トマト TOMJPF00001 TOMJPG1056-1 TOMJPG1450-1 TOMJPG2484-1 TOMJPG4816 TOMJPG5605 TOMJPW0601-1 TOMJPE8506