RRC ID |
538
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著者 |
Ote M, Mita K, Kawasaki H, Daimon T, Kobayashi M, Shimada T.
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タイトル |
Identification of molting fluid carboxypeptidase A (MF-CPA) in Bombyx mori.
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ジャーナル |
Comp Biochem Physiol B Biochem Mol Biol
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Abstract |
Using microarray analyses, we identified carboxypeptidase A (MF-CPA), which was induced during pupal ecdysis in the wing discs of Bombyx mori. Here, we report the functional characterization of MF-CPA. MF-CPA has amino acid sequence similarities with the proteins in the carboxypeptidase A/B subfamily, from human to nematode. The MF-CPA gene is expressed during the molting periods in the epithelial tissues. MF-CPA is detected in the molting fluid, which fills the space between the old and new cuticle during molting. By Western blot analysis, we show that MF-CPA is secreted as a zymogen and processed in the molting fluid. Recombinant MF-CPA expressed in the insect cells has carboxypeptidase A activity. We propose that MF-CPA degrades the proteins from the old cuticle during the molting periods and contributes to recycling of the amino acids.
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巻・号 |
141(3)
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ページ |
314-22
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公開日 |
2005-7-1
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DOI |
10.1016/j.cbpc.2005.04.005
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PII |
S1096-4959(05)00081-3
|
PMID |
15936966
|
MeSH |
Amino Acid Sequence
Animals
Baculoviridae
Bombyx / enzymology*
Bombyx / genetics
Bombyx / growth & development*
Carboxypeptidases A / genetics
Carboxypeptidases A / immunology
Carboxypeptidases A / metabolism*
Chromatography, High Pressure Liquid
Gene Expression Regulation, Developmental*
Larva / enzymology
Molecular Sequence Data
Molting / physiology*
Phylogeny
Rabbits
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
|
IF |
2.219
|
引用数 |
32
|
WOS 分野
|
ZOOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
カイコ |
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