RRC ID 538
著者 Ote M, Mita K, Kawasaki H, Daimon T, Kobayashi M, Shimada T.
タイトル Identification of molting fluid carboxypeptidase A (MF-CPA) in Bombyx mori.
ジャーナル Comp Biochem Physiol B Biochem Mol Biol
Abstract Using microarray analyses, we identified carboxypeptidase A (MF-CPA), which was induced during pupal ecdysis in the wing discs of Bombyx mori. Here, we report the functional characterization of MF-CPA. MF-CPA has amino acid sequence similarities with the proteins in the carboxypeptidase A/B subfamily, from human to nematode. The MF-CPA gene is expressed during the molting periods in the epithelial tissues. MF-CPA is detected in the molting fluid, which fills the space between the old and new cuticle during molting. By Western blot analysis, we show that MF-CPA is secreted as a zymogen and processed in the molting fluid. Recombinant MF-CPA expressed in the insect cells has carboxypeptidase A activity. We propose that MF-CPA degrades the proteins from the old cuticle during the molting periods and contributes to recycling of the amino acids.
巻・号 141(3)
ページ 314-22
公開日 2005-7-1
DOI 10.1016/j.cbpc.2005.04.005
PII S1096-4959(05)00081-3
PMID 15936966
MeSH Amino Acid Sequence Animals Baculoviridae Bombyx / enzymology* Bombyx / genetics Bombyx / growth & development* Carboxypeptidases A / genetics Carboxypeptidases A / immunology Carboxypeptidases A / metabolism* Chromatography, High Pressure Liquid Gene Expression Regulation, Developmental* Larva / enzymology Molecular Sequence Data Molting / physiology* Phylogeny Rabbits Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid
IF 2.219
引用数 32
WOS 分野 ZOOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
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