RRC ID 36722
著者 Osuki KI, Hashimoto S, Suzuki A, Araragi M, Takahara A, Kurosawa M, Kucho KI, Higashi S, Abe M, Uchiumi T.
タイトル Gene expression and localization of a β-1,3-glucanase of Lotus japonicus.
ジャーナル J Plant Res
Abstract Phytohormone abscisic acid (ABA) inhibits root nodule formation of leguminous plants. LjGlu1, a β-1,3-glucanase gene of Lotus japonicus, has been identified as an ABA responsive gene. RNA interference of LjGlu1 increased nodule number. This suggests that LjGlu1 is involved in the regulation of nodule formation. Host legumes control nodule number by autoregulation of nodulation (AON), in which the presence of existing root nodules inhibits further nodulation. For further characterization of LjGlu1, we focused on the expression of LjGlu1 in relation to AON. In a split-root system, LjGlu1 expression peaked when AON was fully induced. Hairy roots transformed with LjCLE-RS1, a gene that induces AON, were generated. Expression of LjGlu1 was greater in the transgenic roots than in untransformed roots. LjGlu1 was not induced in a hypernodulating mutant inoculated with Mesorhizobium loti. These results suggest that the expression of LjGlu1 is involved in the system of AON. However, neither hypernodulation nor enlarged nodulation zone was observed on the transgenic hairy roots carrying LjGlu1-RNAi, suggesting that LjGlu1 is not a key player of AON. Recombinant LjGlu1 showed endo-β-1,3-glucanase activity. LjGlu1-mOrange fusion protein suggested that LjGlu1 associated with M. loti on the root hairs. Exogenous β-1,3-glucanase inhibited infection thread formation by both the wild type and the mutant, and nodule numbers were reduced. These results suggest that LjGlu1 is expressed in response to M. loti infection and functions outside root tissues, resulting in the inhibition of infection.
巻・号 129(4)
ページ 749-758
公開日 2016-7-1
DOI 10.1007/s10265-016-0811-6
PII 10.1007/s10265-016-0811-6
PMID 26951113
MeSH Gene Expression Regulation, Plant* Glucan 1,3-beta-Glucosidase / genetics* Glucan 1,3-beta-Glucosidase / metabolism Lotus / enzymology* Lotus / genetics* Lotus / microbiology Mesorhizobium / physiology Mutation / genetics Plant Root Nodulation / genetics Plants, Genetically Modified RNA Interference Recombinant Proteins / metabolism Root Nodules, Plant / metabolism Root Nodules, Plant / microbiology Transformation, Genetic
IF 2.185
引用数 1
WOS 分野 PLANT SCIENCES
リソース情報
ミヤコグサ・ダイズ Miyakojima MG-20