RRC ID 892
著者 Liu H, Kawabe A, Matsunaga S, Murakawa T, Mizukami A, Yanagisawa M, Nagamori E, Harashima S, Kobayashi A, Fukui K.
タイトル Obtaining transgenic plants using the bio-active beads method.
ジャーナル J Plant Res
Abstract Several methods of transformation are currently available for delivering exogenous DNA into animal and plant cells. In this study, a novel and efficient transformation system for DNA delivery/expression with a capacity to transport DNA of high molecular weight was developed. This system can overcome the shortcomings of traditional transformation methods such as Agrobacterium-mediated transformation, particle bombardment, and the electroporation method. The method developed in this study uses calcium alginate micro beads to immobilize DNA molecules in combination with polyethylene glycol treatment. In addition, it is simple and low-cost, and requires limited equipment. Using this method, we have successfully transformed tobacco plants, screening by kanamycin resistance. The transformed genes in the transformants were confirmed by PCR and Southern hybridization.
巻・号 117(2)
ページ 95-9
公開日 2004-4-1
DOI 10.1007/s10265-003-0141-3
PMID 15108034
MeSH Alginates / pharmacology Culture Media Glucuronic Acid / pharmacology Hexuronic Acids / pharmacology Immobilization Kanamycin / pharmacology Kanamycin Resistance Plant Proteins / genetics Plants, Genetically Modified* Plasmids Polyethylene Glycols / pharmacology Protoplasts Tobacco / genetics* Transformation, Genetic*
IF 2.185
引用数 10
WOS 分野 PLANT SCIENCES
リソース情報
酵母