RRC ID |
17492
|
著者 |
Tashiro-Yamaji J, Shimizu T, Hayashi M, Yamana H, Tanigawa N, Uchiyama K, Kubota T, Yoshida R.
|
タイトル |
Specific binding of HLA-B44 to human macrophage MHC receptor 1 on monocytes.
|
ジャーナル |
Gene
|
Abstract |
Allograft (H-2D(d)K(d))-induced macrophages (AIM) in C57BL/6 (H-2D(b)K(b)) mice exhibit major histocompatibility complex (MHC) haplotype-specific killing of allografts in a macrophage MHC receptor 1 (MMR1; for H-2D(d))- and MMR2 (for H-2K(d))-dependent manner. Recently, we showed HLA-B62 to be a ligand for the human homologue of mouse MMR2. In the present study, we isolated a cDNA encoding the human homologue of mouse MMR1 and found HLA-B44 to be the sole ligand specific for the human MMR1 by using beads that had been conjugated with 80 kinds of HLA proteins. Flow cytometric analyses revealed that HLA-B44-conjugated beads are specifically bound to HEK293T cells expressing human MMR1, that HLA-B44 tetramers are bound to the human MMR1-transfected HEK293T cells with a dissociation constant of 3.0×10(-9) M, and that the interaction was completely inhibited by the addition of R15 monoclonal antibody specific for mouse MMR1. The MMR1 cDNA (1537-bp) encoded a 473-amino acid polypeptide and was expressed at least in part in the brain and peripheral blood mononuclear cells (PBMCs) or monocytes, but not in granulocytes or lymphocytes. PBMCs from 7 non-H-2D(d) (non-self), but none from 5 H-2D(d) (self), in-bred mice expressed mouse MMR1 specific for H-2D(d). In contrast, PBMCs from none of the 16 human volunteers expressed HLA-B44; whereas those from only 3 of these 16 volunteers expressed human MMR1. These results reveal that human MMR1 on monocytes is a novel receptor specific for HLA-B44.
|
巻・号 |
501(2)
|
ページ |
127-34
|
公開日 |
2012-6-15
|
DOI |
10.1016/j.gene.2012.04.020
|
PII |
S0378-1119(12)00433-7
|
PMID |
22525042
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Brain / immunology
HEK293 Cells
HLA-B44 Antigen / chemistry
HLA-B44 Antigen / immunology*
Humans
Leukocytes, Mononuclear / immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Sequence Data
Monocytes / immunology*
Receptors, Immunologic / chemistry
Receptors, Immunologic / genetics
Receptors, Immunologic / immunology*
|
IF |
2.984
|
引用数 |
6
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
実験動物マウス |
RBRC00524 |