RRC ID 45683
著者 Bhat JM, Pan J, Hutter H.
タイトル PLR-1, a putative E3 ubiquitin ligase, controls cell polarity and axonal extensions in C. elegans.
ジャーナル Dev Biol
Abstract During embryonic development neurons differentiate and extend axons and dendrites that have to reach their appropriate targets. In Caenorhabditis elegans the AVG neuron is the first neuron to extend an axon during the establishment of the ventral nerve cord, the major longitudinal axon tract in the animal. In genetic screens we isolated alleles of plr-1, which caused polarity reversals of the AVG neuron as well as outgrowth and navigation defects of the AVG axon. In addition plr-1 mutants show outgrowth defects in several other classes of neurons as well as the posterior excretory canals. plr-1 is predicted to encode a transmembrane E3 ubiquitin ligase and is widely expressed in the animal including the AVG neuron and the excretory cell. plr-1 has recently been shown to negatively regulate Wnt signalling by removing Wnt receptors from the cell surface. We observed that mutations in a gene reducing Wnt signalling as well as mutations in unc-53/NAV2 and unc-73/Trio suppress the AVG polarity defects in plr-1 mutants, but not the defects seen in other cells. This places plr-1 in a Wnt regulation pathway, but also suggests that plr-1 has Wnt independent functions and interacts with unc-53 and unc-73 to control cell polarity.
巻・号 398(1)
ページ 44-56
公開日 2015-2-1
DOI 10.1016/j.ydbio.2014.11.008
PII S0012-1606(14)00585-5
PMID 25448694
MeSH Alleles Amino Acid Sequence Animals Axons / physiology* Caenorhabditis elegans / enzymology Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Membrane / metabolism Cell Movement Cell Polarity* Gene Expression Regulation, Developmental* Green Fluorescent Proteins / metabolism Microfilament Proteins / metabolism Molecular Sequence Data Nerve Tissue Proteins / metabolism Neurons / metabolism Phenotype Protein Structure, Tertiary Signal Transduction Ubiquitin-Protein Ligases / physiology* Wnt Proteins / metabolism
IF 2.896
引用数 5
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm2957 tm2975