RRC ID 50384
著者 Ikebe R, Kuwabara Y, Chikada T, Niki H, Shiomi D.
タイトル The periplasmic disordered domain of RodZ promotes its self-interaction in Escherichia coli.
ジャーナル Genes Cells
Abstract Rod shape of bacterial cells such as Escherichia coli is mainly regulated by a supramolecular complex called elongasome including MreB actin. Deletion of the mreB gene in rod-shaped bacterium E. coli results in round-shaped cells. RodZ was isolated as a determinant of rod shape in E. coli, Caulobacter crescentus and Bacillus subtilis and it has been shown to be an interaction partner and a regulator of assembly of MreB through its cytoplasmic domain. As opposed to functions of the N-terminal cytoplasmic domain of RodZ, functions of the C-terminal periplasmic domain including a disordered region are still unclear. To understand it, we adopted an in vivo photo-cross-linking assay to analyze interaction partners to identify proteins which interact with RodZ via its periplasmic domain, finding that the RodZ self-interacts in the periplasmic disordered domain. Self-interaction of RodZ was affected by MreB actin. Deletion of this region resulted in aberrant cell shape. Our results suggest that MreB binding to the cytoplasmic domain of RodZ causes structural changes in the disordered periplasmic domain of RodZ. We also found that the disordered domain of RodZ contributes to fine-tune rod shape in E. coli.
巻・号 23(4)
ページ 307-317
公開日 2018-4-1
DOI 10.1111/gtc.12572
PMID 29480545
MeSH Actins / metabolism Cell Shape Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Escherichia coli / cytology* Escherichia coli / growth & development Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Periplasm / metabolism* Protein Multimerization
IF 1.655
引用数 5
リソース情報
原核生物(大腸菌) ME9807 ME9808 ME9809 ME9810 ME9811