RRC ID 4805
著者 Geu-Flores F, Olsen CE, Halkier BA.
タイトル Towards engineering glucosinolates into non-cruciferous plants.
ジャーナル Planta
Abstract Glucosinolates are amino acid-derived secondary metabolites present in cruciferous plants. Glucosinolates and their hydrolysis products are involved in defence against insects and pathogens, but are also known for their characteristic flavor and their cancer-preventive and antibacterial properties. This wide range of bioactivities has prompted a desire to engineer glucosinolates into non-cruciferous plants. We report the one-step transfer of the last three steps of the benzylglucosinolate pathway (comprising the C-S lyase, glycosyltransferase and sulfotransferase) from Arabidopsis to tobacco. This was achieved using an expression construct consisting of a single 2A polycistronic open reading frame, which allowed the expression of the three coding-sequences from a single promoter. When compared to wildtype plants, transgenic tobacco lines showed increased ability to convert the intermediate phenylacetothiohydroxamate to benzylglucosinolate upon in vivo feeding. Enzymatic assays using plant extracts demonstrated that the individual activities required for this conversion were enhanced in the transgenic plants. The relatively high conversion by wildtype plants in feeding assays supports the hypothesis that the last part of the glucosinolate pathway was recruited from existing detoxification reactions. Immunoblots confirmed that individual proteins were being successfully produced from the 2A polycistronic open reading frame, albeit fusion proteins could also be detected. In summary, we transferred the last three steps of the benzylglucosinolate pathway to tobacco as a first step towards engineering glucosinolates into non-cruciferous plants.
巻・号 229(2)
ページ 261-70
公開日 2009-1-1
DOI 10.1007/s00425-008-0825-y
PMID 18830705
MeSH Biological Assay Blotting, Western Brassicaceae / metabolism* Genetic Engineering* Glucosinolates / metabolism* Glucosyltransferases / metabolism Open Reading Frames / genetics Plants, Genetically Modified Protein Biosynthesis Sulfotransferases / metabolism Thiocyanates / metabolism Thioglucosides / metabolism Transcription, Genetic Transformation, Genetic
IF 3.39
引用数 43
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda00614 pda01219