RRC ID 32790
著者 Rüegger S, Miki TS, Hess D, Großhans H.
タイトル The ribonucleotidyl transferase USIP-1 acts with SART3 to promote U6 snRNA recycling.
ジャーナル Nucleic Acids Res
Abstract The spliceosome is a large molecular machine that serves to remove the intervening sequences that are present in most eukaryotic pre-mRNAs. At its core are five small nuclear ribonucleoprotein complexes, the U1, U2, U4, U5 and U6 snRNPs, which undergo dynamic rearrangements during splicing. Their reutilization for subsequent rounds of splicing requires reversion to their original configurations, but little is known about this process. Here, we show that ZK863.4/USIP-1 (U Six snRNA-Interacting Protein-1) is a ribonucleotidyl transferase that promotes accumulation of the Caenorhabditis elegans U6 snRNA. Endogenous USIP-1-U6 snRNA complexes lack the Lsm proteins that constitute the protein core of the U6 snRNP, but contain the U6 snRNP recycling factor SART3/B0035.12. Furthermore, co-immunoprecipitation experiments suggest that SART3 but not USIP-1 occurs also in a separate complex containing both the U4 and U6 snRNPs. Based on this evidence, genetic interaction between usip-1 and sart-3, and the apparent dissociation of Lsm proteins from the U6 snRNA during spliceosome activation, we propose that USIP-1 functions upstream of SART3 to promote U6 snRNA recycling.
巻・号 43(6)
ページ 3344-57
公開日 2015-3-31
DOI 10.1093/nar/gkv196
PII gkv196
PMID 25753661
PMC PMC4381082
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Genes, Helminth Models, Biological Nucleotide Transport Proteins / genetics Nucleotide Transport Proteins / metabolism RNA Interference RNA Nucleotidyltransferases / genetics RNA Nucleotidyltransferases / metabolism RNA Stability RNA, Helminth / genetics RNA, Helminth / metabolism* RNA, Small Nuclear / genetics RNA, Small Nuclear / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Recombinant Proteins / genetics Recombinant Proteins / metabolism Ribonucleoprotein, U4-U6 Small Nuclear / genetics Ribonucleoprotein, U4-U6 Small Nuclear / metabolism
IF 11.502
引用数 14
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm1897