| RRC ID |
6291
|
| 著者 |
Wang H, Zhu Y, Fujioka S, Asami T, Li J, Li J.
|
| タイトル |
Regulation of Arabidopsis brassinosteroid signaling by atypical basic helix-loop-helix proteins.
|
| ジャーナル |
Plant Cell
|
| Abstract |
Basic helix-loop-helix (bHLH) proteins are highly conserved transcription factors critical for cell proliferation and differentiation. Recent studies have implicated bHLH proteins in many plant signaling processes, including brassinosteroid (BR) signaling. Here, we report identification of two families of atypical bHLH proteins capable of modulating BR signaling. We found that activation-tagged bri1 suppressor 1-Dominant (atbs1-D), previously identified as a dominant suppressor of a weak BR receptor mutant bri1-301, was caused by overexpression of a 93-amino acid atypical bHLH protein lacking amino acids critical for DNA binding. Interestingly, atbs1-D only suppresses weak BR mutants, while overexpression of a truncated ATBS1 lacking the basic motif also rescues bri1-301, suggesting that ATBS1 likely stimulates BR signaling by sequestering negative BR signaling components. A yeast two-hybrid screen using ATBS1 as bait discovered four ATBS1-Interacting Factors (AIFs) that are members of another atypical bHLH protein subfamily. AIF1 exhibits an overlapping expression pattern with ATBS1 and its homologs and interacts with ATBS1 in vitro and in vivo. AIF1 overexpression nullifies the suppressive effect of atbs1-D on bri1-301 and results in dwarf transgenic plants resembling BR mutants. By contrast, silencing of AIF1 partially suppressed the bri1-301 phenotype. Our results suggested that plants use these atypical bHLH proteins to regulate BR signaling.
|
| 巻・号 |
21(12)
|
| ページ |
3781-91
|
| 公開日 |
2009-12-1
|
| DOI |
10.1105/tpc.109.072504
|
| PII |
tpc.109.072504
|
| PMID |
20023194
|
| PMC |
PMC2814491
|
| MeSH |
Amino Acid Sequence
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Basic Helix-Loop-Helix Transcription Factors / genetics
Basic Helix-Loop-Helix Transcription Factors / metabolism*
Gene Expression Regulation, Plant
Gene Silencing
Molecular Sequence Data
Plant Growth Regulators / metabolism*
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
RNA, Plant / genetics
Sequence Alignment
Signal Transduction*
Steroids / metabolism
|
| IF |
9.618
|
| 引用数 |
111
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda |
