RRC ID 36040
著者 Lv X, Pan C, Zhang Z, Xia Y, Chen H, Zhang S, Guo T, Han H, Song H, Zhang L, Zhao Y.
タイトル SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis.
ジャーナル Development
Abstract SUMO (Small ubiquitin-related modifier) modification (SUMOylation) is a highly dynamic post-translational modification (PTM) that plays important roles in tissue development and disease progression. However, its function in adult stem cell maintenance is largely unknown. Here, we report the function of SUMOylation in somatic cyst stem cell (CySC) self-renewal in adult Drosophila testis. The SUMO pathway cell-autonomously regulates CySC maintenance. Reduction of SUMOylation promotes premature differentiation of CySCs and impedes the proliferation of CySCs, which leads to a reduction in the number of CySCs. Consistent with this, CySC clones carrying a mutation of the SUMO-conjugating enzyme are rapidly lost. Furthermore, inhibition of the SUMO pathway phenocopies disruption of the Hedgehog (Hh) pathway, and can block the proliferation of CySCs induced by Hh activation. Importantly, the SUMO pathway directly regulates the SUMOylation of Hh pathway transcription factor Cubitus interruptus (Ci), which is required for promoting CySC proliferation. Thus, we conclude that SUMO directly targets the Hh pathway and regulates CySC maintenance in adult Drosophila testis.
巻・号 143(10)
ページ 1655-62
公開日 2016-5-15
DOI 10.1242/dev.130773
PII dev.130773
PMID 27013244
MeSH Aging / physiology* Animals Cell Differentiation Cell Proliferation Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / metabolism Epistasis, Genetic Hedgehog Proteins / metabolism* Male Signal Transduction* Small Ubiquitin-Related Modifier Proteins / metabolism* Stem Cells / cytology* Stem Cells / metabolism* Sumoylation Testis / cytology* Testis / metabolism
IF 5.611
引用数 9
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 2411R-1