RRC ID 37062
著者 Arai Y, Yamamoto T, Minamikawa T, Takamatsu T, Nagai T.
タイトル Spectral fingerprinting of individual cells visualized by cavity-reflection-enhanced light-absorption microscopy.
ジャーナル PLoS One
Abstract The absorption spectrum of light is known to be a "molecular fingerprint" that enables analysis of the molecular type and its amount. It would be useful to measure the absorption spectrum in single cell in order to investigate the cellular status. However, cells are too thin for their absorption spectrum to be measured. In this study, we developed an optical-cavity-enhanced absorption spectroscopic microscopy method for two-dimensional absorption imaging. The light absorption is enhanced by an optical cavity system, which allows the detection of the absorption spectrum with samples having an optical path length as small as 10 μm, at a subcellular spatial resolution. Principal component analysis of various types of cultured mammalian cells indicates absorption-based cellular diversity. Interestingly, this diversity is observed among not only different species but also identical cell types. Furthermore, this microscopy technique allows us to observe frozen sections of tissue samples without any staining and is capable of label-free biopsy. Thus, our microscopy method opens the door for imaging the absorption spectra of biological samples and thereby detecting the individuality of cells.
巻・号 10(5)
ページ e0125733
公開日 2015-1-1
DOI 10.1371/journal.pone.0125733
PII PONE-D-14-56095
PMID 25950513
PMC PMC4423951
MeSH Animals COS Cells Chlorocebus aethiops HeLa Cells Humans Light Mice Microscopy / methods* NIH 3T3 Cells PC12 Cells Principal Component Analysis Rats Single-Cell Analysis / methods*
IF 2.74
引用数 2
WOS 分野 MULTIDISCIPLINARY SCIENCES
リソース情報
ヒト・動物細胞 Colon-26(RCB2657) HeLa COS-7(RCB0539) NIH3T3 201B7(HPS0063) PC-12(RCB0009)