RRC ID |
37062
|
著者 |
Arai Y, Yamamoto T, Minamikawa T, Takamatsu T, Nagai T.
|
タイトル |
Spectral fingerprinting of individual cells visualized by cavity-reflection-enhanced light-absorption microscopy.
|
ジャーナル |
PLoS One
|
Abstract |
The absorption spectrum of light is known to be a "molecular fingerprint" that enables analysis of the molecular type and its amount. It would be useful to measure the absorption spectrum in single cell in order to investigate the cellular status. However, cells are too thin for their absorption spectrum to be measured. In this study, we developed an optical-cavity-enhanced absorption spectroscopic microscopy method for two-dimensional absorption imaging. The light absorption is enhanced by an optical cavity system, which allows the detection of the absorption spectrum with samples having an optical path length as small as 10 μm, at a subcellular spatial resolution. Principal component analysis of various types of cultured mammalian cells indicates absorption-based cellular diversity. Interestingly, this diversity is observed among not only different species but also identical cell types. Furthermore, this microscopy technique allows us to observe frozen sections of tissue samples without any staining and is capable of label-free biopsy. Thus, our microscopy method opens the door for imaging the absorption spectra of biological samples and thereby detecting the individuality of cells.
|
巻・号 |
10(5)
|
ページ |
e0125733
|
公開日 |
2015-1-1
|
DOI |
10.1371/journal.pone.0125733
|
PII |
PONE-D-14-56095
|
PMID |
25950513
|
PMC |
PMC4423951
|
MeSH |
Animals
COS Cells
Chlorocebus aethiops
HeLa Cells
Humans
Light
Mice
Microscopy / methods*
NIH 3T3 Cells
PC12 Cells
Principal Component Analysis
Rats
Single-Cell Analysis / methods*
|
IF |
2.74
|
引用数 |
2
|
WOS 分野
|
MULTIDISCIPLINARY SCIENCES
|
リソース情報 |
ヒト・動物細胞 |
Colon-26(RCB2657)
HeLa
COS-7(RCB0539)
NIH3T3
201B7(HPS0063)
PC-12(RCB0009) |