RRC ID 12628
著者 Furune T, Hashimoto K, Ishiguro J.
タイトル Characterization of a fission yeast P(5)-type ATPase homologue that is essential for Ca(2+)/Mn(2+ )homeostasis in the absence of P(2)-type ATPases.
ジャーナル Genes Genet Syst
Abstract In the fission yeast Schizosaccharomyces pombe, three P-type ATPases, namely Cta4p, Pmr1p, and Pmc1p, have been shown to be essential for Ca(2+) homeostasis and are required for specific cellular functions as well. Here, we show that the simultaneous deletion of pmc1(+) and SPAC29A4.19c, which encodes a putative P(5)-type ATPase, causes a hypersensitive growth to either high concentrations of Ca(2+) in a medium, or the antiarrhythmic drug amiodarone, which has been known to cause a disruption of Ca(2+) homeostasis. On the other hand, simultaneous deletion of pmr1(+) and SPAC29A4.19c causes a hypersensitive growth to Mn(2+) depletion in a medium. The green fluorescent protein-tagged SPAC29A4.19c protein reveals a typical localization pattern of the Golgi proteins, but the SPAC29A4.19c protein is not exchangeable in function with Pmr1p, which is required for Ca(2+)/Mn(2+) homeostasis in secretory pathways. These results suggest that the putative P(5)-type ATPase encoded by SPAC29A4.19c is essential for Ca(2+) and Mn(2+ )homeostasis in the absence of P(2)-type ATPases, Pmc1p or Pmr1p, respectively. According to the precedent nomenclature of calcium/cation transporting ATPase in fission yeast, SPAC29A4.19 was named cta5(+) in this study.
巻・号 83(5)
ページ 373-81
公開日 2008-10-1
DOI 10.1266/ggs.83.373
PII JST.JSTAGE/ggs/83.373
PMID 19168988
MeSH Calcium / metabolism* Calcium-Transporting ATPases / genetics* Calcium-Transporting ATPases / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Homeostasis Manganese / metabolism* Proton-Translocating ATPases / genetics* Proton-Translocating ATPases / metabolism Schizosaccharomyces / enzymology* Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism
IF 0.917
引用数 11
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 JY741 JY746 ?