RRC ID 52510
著者 Boström J, Sramkova Z, Salašová A, Johard H, Mahdessian D, Fedr R, Marks C, Medalová J, Souček K, Lundberg E, Linnarsson S, Bryja V, Sekyrova P, Altun M, Andäng M.
タイトル Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.
ジャーナル PLoS One
Abstract The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.
巻・号 12(12)
ページ e0188772
公開日 2017-12-11
DOI 10.1371/journal.pone.0188772
PII PONE-D-17-26058
PMID 29228002
PMC PMC5724894
MeSH Algorithms Cell Cycle / genetics* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Line, Tumor Humans Neoplasms / genetics Neoplasms / metabolism* Neoplasms / pathology Transcription Factors / metabolism* Transcriptome*
IF 2.74
引用数 5
リソース情報
ヒト・動物細胞 HeLa/Fucci(RCB2812)