RRC ID 4412
著者 Yamada K, Hirota K, Mizuno K, Shibata T, Ohta K.
タイトル Essential roles of Snf21, a Swi2/Snf2 family chromatin remodeler, in fission yeast mitosis.
ジャーナル Genes Genet Syst
Abstract ATP-dependent chromatin remodelers (ADCRs) convert local chromatin structure into both transcriptional active and repressive state. Recent studies have revealed that ADCRs play diverse regulatory roles in chromosomal events such as DNA repair and recombination. Here we have newly identified a fission yeast gene encoding a Swi2/Snf2 family ADCR. The amino acid sequence of this gene, snf21(+), implies that Snf21 is a fission yeast orthologue of the budding yeast Sth1, the catalytic core of the RSC chromatin remodeling complex. The snf21(+) gene product is a nuclear protein essential to cell viability: the null mutant cells stop growing after several rounds of cell divisions. A temperature sensitive allele of snf21(+), snf21-36 exhibits at non-permissive temperature (34 degrees C) a cell cycle arrest at G2-M phase and defects in chromosome segregation, thereby causing cell elongation, lack of cell growth, and death of some cell population. snf21-36 shows thiabendazole (TBZ) sensitivity even at permissive temperature (25 degrees C). The TBZ sensitivity becomes severer as snf21-36 is combined with the deletion of a centromere-localized Mad2 spindle checkpoint protein. The cell cycle arrest phenotype at 34 degrees C cannot be rescued by the mad2(+) deletion, although it is substantially alleviated at 30 degrees C in mad2Delta. These data suggest that Snf21 plays an essential role in mitosis possibly functioning in centromeric chromatin.
巻・号 83(5)
ページ 361-72
公開日 2008-10-1
DOI 10.1266/ggs.83.361
PII JST.JSTAGE/ggs/83.361
PMID 19168987
MeSH Cell Cycle Proteins / analysis Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Survival Chromatin / metabolism Chromatin Assembly and Disassembly / physiology* Chromosome Segregation DNA Helicases / genetics DNA Helicases / metabolism Mitosis* Mutation Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / analysis Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism*
IF 0.917
引用数 13
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY7708