RRC ID 41197
著者 Chang DH, Rhee MS, Ahn S, Bang BH, Oh JE, Lee HK, Kim BC.
タイトル Faecalibaculum rodentium gen. nov., sp. nov., isolated from the faeces of a laboratory mouse.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel strictly anaerobic strain, ALO17(T), was isolated from mouse faeces and found to produce lactic acid as a major metabolic end product. The isolate was observed to be Gram-stain positive, non-motile, non-spore forming small rods, oxidase and catalase negative, and to form cream-coloured colonies on DSM 104 agar plates. The NaCl range for growth was determined to be 0-2 % (w/v). The isolate was found to grow optimally at 37 °C, with 0.5 % (w/v) NaCl and at pH 7. The cell wall hydrolysates were found to contain ribose as a major sugar. The genomic DNA G+C content was determined to be 52.3 mol%. A phylogenetic analysis of the 16S rRNA gene sequence revealed that Holdemanella biformis DSM 3989(T), Faecalicoccus pleomorphus ATCC 29734(T), Faecalitalea cylindroides ATCC 27803(T), and Allobaculum stercoricanis DSM 13633(T) are closely related to the isolate (87.4, 87.3, 86.9 and 86.9 % sequence similarity), respectively. The major cellular fatty acids (>10 %) of the isolate were identified as C18:1 cis 9 FAME (36.9 %), C16:0 FAME (33.7 %) and C18:0 FAME (13.2 %). In contrast to the tested reference strains, C20:0 FAME (4.0 %) was detected only in strain ALO17(T) whilst C16:0 DMA was absent. The isolate also differed in its substrate oxidation profiles from the reference strains by being positive for D-melibiose and stachyose but negative for N-acetyl-D-galactosamine and 3-methyl-D-glucose. On the basis of polyphasic taxonomic evidence from this study, the isolate is concluded to belong to a novel genus within the family Erysipelothricaceae. We propose the name Faecalibaculum rodentium gen. nov., sp. nov. to accommodate strain ALO17(T) (=KCTC 15484(T) = JCM 30274(T)) as the type strain.
巻・号 108(6)
ページ 1309-1318
公開日 2015-12-1
DOI 10.1007/s10482-015-0583-3
PII 10.1007/s10482-015-0583-3
PMID 26349481
MeSH Anaerobiosis Animals Base Composition Carbohydrates / analysis Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Feces / microbiology* Firmicutes / classification* Firmicutes / genetics Firmicutes / isolation & purification* Firmicutes / physiology Hydrogen-Ion Concentration Lactic Acid / metabolism Mice Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Temperature
IF 2.674
引用数 21
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30274