RRC ID 4419
著者 Nakamura S, Saito D, Tanaka M.
タイトル Generation of transgenic medaka using modified bacterial artificial chromosome.
ジャーナル Dev Growth Differ
Abstract The availability of bacterial artificial chromosome (BAC) offers a good genomic platform for a targeted integration of an exogenous gene by a homologous recombination system in Escherichia coli. In combination with microinjection technology, this system allows for the analysis of various aspects of biological phenomena occurring in vivo using Japanese medaka fish (Oryzias latipes). Here we describe a streamlined procedure for selecting BAC clones based on the medaka University of Tokyo genome browser (UTGB), followed by rapid modification with enhanced green fluorescent protein (EGFP) or DsRed fragments for transgenic analysis in medaka. Experimental procedures for BAC DNA preparation, microinjection of medaka embryos and screening of resulting transgenic medaka carrying EGFP/DsRed modified BAC clones are also described.
巻・号 50(6)
ページ 415-9
公開日 2008-8-1
DOI 10.1111/j.1440-169X.2008.01027.x
PII DGD1027
PMID 18422685
MeSH Animals Animals, Genetically Modified* Chromosomes, Artificial, Bacterial* Developmental Biology / methods Escherichia coli Genetic Techniques Genome Green Fluorescent Proteins / metabolism Models, Genetic Oryzias / genetics* Recombination, Genetic Transgenes
IF 1.723
引用数 35
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
メダカ BAC clones (golwb014_G01)